马立克氏病病毒Rispens株B抗原基因的克隆及其重组鸡痘病毒的构建  

CLONING OF GLYCOPROTEIN B GENE FROM STRAIN RISPENS OF MAREK'S DISEASE VIRUS AND CONSTRUCTION OF RECOMBINANT FOWLPOX VIRUS

作　　者：邢力[1] 彭大新[1] 朱爱华 刘秀梵[1] 张如宽[1] 

机构地区：[1]扬州大学农学院动物医学系

出　　处：《微生物学报》1999年第2期164-167,共4页Acta Microbiologica Sinica

基　　金：国家863计划;江苏省自然科学基金

摘　　要：马立克氏病病毒（Ｍａｒｅｋ’ｓＤｉｓｅａｓｅＶｉｒｕｓ，ＭＤＶ）是马立克氏病（ＭＤ）的病原，是第一个能用实验证明具有致肿瘤作用的疱疹病毒，也是研究病毒性肿瘤特别是疱疹病毒肿瘤的理想的实验模型。ＭＤ还是第一个广泛使用疫苗预防的肿瘤性病毒病。应用免疫扩散...Purified DNAs from Chicken Embryo Fibroblast (CEF) cultures infected with MDV strain Rispens were used as templates. Specific fragment with the size of about 2.9kb was successfully amplified through Polymerase Chain Reaction(PCR)and identified to be gB gene of MDV by dot blot hybridization with a digoxigenin labelled MDV gB specific oligonucleotide probe. The gB gene from strain Rispens was cloned into pUC19 and FPV insertion vector pFG1175 1 to construct plasmid pMGB and pFGBR1775 1 respectively. DOSPER liposome mediated transfection with insertion vector DNA pFGBR1175 1 was performed on CEF monolayers infected with FPV 3-4 h earlier. Recombinant FPV was clone purified. Immunofluorescence Assay(IFA) showed that MDV gB gene had been expressed in FPV.

关 键 词：马立克氏病病毒 糖蛋白B抗原 重组鸡痘病毒 

分 类 号：S852.65[农业科学—基础兽医学]