A组轮状病毒G4型地方株VP7基因在杆状病毒系统中的表达  

EXPRESSION OF VP7 FROM A GROUP A ROTAVIRUS G4 FIELD STRAIN IN BACULOVIRUS SYSTEM

作　　者：何湘君[1] 钱渊[1] 刘军[1] 李国华[1] 靖宇[1] 

机构地区：[1]首都儿科研究所北京市感染与免疫中心实验室

出　　处：《微生物学报》1999年第2期168-170,共3页Acta Microbiologica Sinica

基　　金：国家自然科学基金;卫生部科研基金;北京市自然科学基金

摘　　要：Ａ组轮状病毒是导致婴幼儿重症腹泻的最主要病毒病原，位于病毒外壳上的ＶＰ４和ＶＰ７蛋白具有中和抗原活性，与病毒的毒力及免疫保护性有关。ＶＰ７是外壳上的主要糖蛋白，根据其抗原性的不同，可将Ａ组轮状病毒划分出不同的血清型（Ｇ１～Ｇ１４）［１］，而对疫苗的研...The full length of G4 type VP7 cDNA was amplified by reverse transcription and polymerase chain reaction (RT PCR) from a group A rotavirus field strain CR117 circulated in Beijing. The amplified cDNA was first cloned into pTZ18. Sequence analysis showed 96.9% homology of the deduced amino acid between VP7 of CR117 and ST3. Then the VP7 cDNA of CR117 was subcloned into the transfer vector pBacPAK8 downstream of the polyhedrin promotor. Insect cells were cotransfected with recombinant plasmid pBacPAK8 CR117 and Bsu36I digested BacPAK6 viral DNA. Recombinant baculovirs containing CR117 VP7 gene was obtained by plaque screen and hybrydization identification. Specific VP7 with three different molecular weight was detectedby Western blot using hyperimmune serum against Rotavirus strain ST3 (G4 type) when expressed in insect cells.

关 键 词：轮状病毒 VP7 杆状病毒载体 蛋白表达 

分 类 号：R373.2[医药卫生—病原生物学]