BMPR-IB基因作为影响蒙古羊双羔性状候选基因的研究  

Study on the BMPR-IB gene as candidate gene for fecundity in Mongolian sheep double-lambs group

作　　者：杨燕燕[1,2] 田瑛[1] 达来[1] 曹贵方[2] 邵凯[1] 苏和[1] 青格勒[1] 乌达巴拉[1] 周璇[1] 

机构地区：[1]内蒙古农牧业科学院畜牧研究所,内蒙古呼和浩特010030 [2]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010010

出　　处：《黑龙江畜牧兽医》2010年第6期1-4,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：农业部"948"项目(2006-G45);内蒙古自然基金项目(2009MS0413)

摘　　要：为了探索多胎候选基因BMPR-IB与蒙古羊双羔群体繁殖性状之间的关系,试验采用聚合酶链式反应-限制性片段长度多态性分子标记技术(PCR-RFLP),以蒙古羊单羔群体(18只)、多胎类型小尾寒羊(30只)为对照组对蒙古羊双羔群体(50只)进行了BMPR-IB基因的遗传多态性分析。结果表明:蒙古羊双羔群体和蒙古羊单羔群体中都不存在BMPR-IB基因的FecB(A746G)突变,说明BMPR-IB基因不是影响蒙古羊双羔性状的主效基因;而在多胎品种小尾寒羊中检测到了相应的突变,发现了3种基因型,其突变纯合子(BB)、杂合子(B+)和野生型(++)的基因型频率分别是0.100,0.733,0.167,将其与产羔数进行关联分析,结果BB基因型母羊和B+基因型母羊平均产羔数差异极显著(P<0.01);经x2适合性检验,小尾寒羊该基因位点显著偏离Hardy-Weinberg平衡(P<0.05),由此推断BMPR-IB基因是控制小尾寒羊多胎性能的主效基因。The experiment adopted polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） molecular markers technique,and set the single-lamb Mongolian sheep group （18） and multiple births type of small tail Han sheep （30） two contrast groups,analyzed genetic polymorphism of the BMPR-IB gene in Mongolian sheep double-lambs groups （50）,（To explore the relationship between the fecundity candidate genes BMPR-IB and the reproductive traits of Mongolian sheep double-lambs group.） The results showed that the BMPR-IB gene FecB mutation was absent in double-lambs Mongolian sheep group and single-lamb Mongolian sheep group,so BMPR-IB gene wasn’t the major gene that impacted high productivity of Mongolian;the mutation was detected in Small tail han sheep breed and found three kinds of genotypes,the homozygous mutant （BB） and heterozy-gous （B＋） and wild-type （＋＋）,the genotype frequencies were 0.100,0.733 and 0.167,associated with litter size analysis it indicated that average litter size between BB genotype ewes and B ＋ genotype ewes was significant difference （P0.01）,by the chi-sequare （x2）test it showed that the gene located in Small tail han sheep was significant deviation from Hardy-Weinberg equilibrium （P0.05）.It can infer that BMPR-IB gene is the Major gene for high fecundity of multiple births of Small tail han sheep.

关 键 词：蒙古羊 小尾寒羊 绵羊 多胎 BMPR-IB基因 

分 类 号：S826.82[农业科学—畜牧学]