妊娠大鼠碘缺乏和甲状腺功能减退对仔鼠海马脑源性神经营养因子表达的影响  

Effects of Iodine Deficiency and Hypothyroidism in Pregnant Rats on Protein Expressions of Brain-Derived Neurotrophic Factor in Hippocampus of Offspring

作　　者：刘万洋[1] 魏薇[1] 董静[1] 王毅[1] 陈杰[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生与环境卫生学教研室,辽宁沈阳110001

出　　处：《环境与健康杂志》2010年第6期471-473,F0003,共4页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(30500400)

摘　　要：目的观察妊娠大鼠碘缺乏和甲状腺功能减退对仔鼠海马脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响。方法将妊娠清洁级Wistar大鼠28只按体重随机分成对照组、碘缺乏组、甲状腺功能减退1、2组,每组7只。自GD6起,碘缺乏组饲以缺碘地区粮食配制的饲料[碘含量为(14.11±1.96)ng/g],饮用自来水;甲状腺功能减退1、2组分别给予5和15mg/L丙基硫尿嘧啶(propylthiouracil,PTU)溶液作为饮用水,饲喂普通饲料[碘含量为(470.50±46.52)ng/g],直至仔鼠出生后第28天(PN28);对照组饮用自来水,饲喂普通饲料。分别于PN14、PN21、PN28和PN42时,每组随机取5只仔鼠,观察海马CA1、CA3和DG区BDNF表达。结果在PN14时,各组仔鼠海马CA1、CA3和DG区BDNF蛋白表达水平间比较,差异均无统计学意义。在PN21时,甲状腺功能减退2组和碘缺乏组仔鼠海马CA1、CA3和DG区BDNF蛋白表达水平低于对照组,差异均有统计学意义(P<0.05);碘缺乏组仔鼠海马CA1区以及甲状腺功能减退2组和碘缺乏组仔鼠海马DG区BDNF蛋白水平低于甲状腺功能减退1组,差异均有统计学意义(P<0.05)。在PN28时,甲状腺功能减退2组和碘缺乏组仔鼠海马CA1、CA3和DG区以及甲状腺功能减退1组仔鼠海马CA3区BDNF蛋白表达水平低于对照组,差异均有统计学意义(P<0.05);甲状腺功能减退2组和碘缺乏组仔鼠海马CA1区BDNF蛋白表达水平低于甲状腺功能减退1组,差异均有统计学意义(P<0.05)。在PN42时,甲状腺功能减退1、2组和碘缺乏组仔鼠海马CA1、CA3和DG区BDNF蛋白表达水平低于对照组,差异均有统计学意义(P<0.05);甲状腺功能减退2组和碘缺乏组仔鼠海马CA1和DG区BDNF蛋白表达水平低于甲状腺功能减退1组,差异均有统计学意义(P<0.05)。结论妊娠大鼠碘缺乏和甲状腺功能减退可降低仔鼠海马BDNF的表达。Objective To study the effects of iodine deficiency and hypothyroidism of pregnant rats on the protein expressions of brain-derived neurotrophic factor （BDNF） in the hippocampus of offspring. Methods The pregnant Wistar rats （n=28） were randomly divided into control group,hypothyroid 1, 2 groups and iodine deficient group （ 7 in each group）. From GD6 till PN28, iodine-deficient group was administered with iodine-deficient diet [iodine content： （14.11±1.96） ng/g] and tap water; hypothyroid 1 and 2 groups were administered with 5 mg/L and 15 mg/L PTU in the drinking water and were fed with normal diet [iodine content： （470.50±46.52） ng/g]. Control group received tap water and normal diet during the study. Five offspring from each group were observed and analyzed for the protein expression of BDNF by immunohistochemistry in the CA1,CA3 and DG regions of hippocampus at PN14,PN21 ,PN28 and PN42. Results At PN14, no significant difference was observed in the protein expression among all four groups. At PN21 ,the expression of BDNF in the CA1, CA3 and DG regions of hippocampus in hypothyroid 2 group and iodine deficient group were significantly lower than those of the control group （P〈0.05）; the expression of BDNF in the CA1 regions of hippoeampus in iodine deficient group and in the DG regions of hippocampus in hypothyroid 2 and iodine deficient group were significantly lower than those of hypothyroid 1 group （P〈0.05）. At PN28, the expression of BDNF in the CA1 ,CA3 and DG regions of hippocampus in hypothyroid 2 group and iodine deficient group and in the CA3 region of hippoeampus in hypothyroid 1 group were significantly lower than those of the control group （P〈0.05）; the expression of BDNF in the CA1 region of hippocampus in hypothyroid 2 group and iodine deficient group were significantly lower than those of hypothyroid 1 group（P〈0.05）. At PN42, the expression of BDNF in the CAI,CA3 and DG regions of hippoeampus in hypothyroid 1 group, hypothyroid 2 group and iodine

关 键 词：碘缺乏 甲状腺功能减退症 海马 脑源性神经营养因子 

分 类 号：R994.6[医药卫生—毒理学]