方斑东风螺肿吻症病原菌的分离鉴定及药敏分析  

Isolation, identification and antibiotic sensitivity analysis of bacterial pathogen from proboscis intumescence disease in Babylonia areolata

作　　者：张新中[1] 文万侥[1] 冯永勤[1] 谢珍玉[1] 周永灿[1] 

机构地区：[1]海南大学海洋学院,海南海口570228

出　　处：《海洋科学》2010年第5期7-12,共6页Marine Sciences

基　　金：教育部新世纪优秀人才支持计划项目(NCET-05-0755);科技部国际合作重点项目(2005DFA30610)

摘　　要：从海南省文昌县某养殖场患病方斑东风螺（Babylonia areolata）的吻、肌肉和肝脏中分离到2株优势菌。人工感染证实其中1株为致病菌，经形态学检测和生化鉴定，该病原菌为鳗弧菌生物变种Ⅰ（Vibrio anguillarum Biovar Ⅰ）。该病原菌对方斑东风螺的LD50值为1．7×10^6cfu／g。药敏试验表明派拉西林、罗红霉素、红霉素、洁霉素、氟哌酸、先锋必、妥布霉素、菌必治、复方新诺明、利福平、复达欣、呋喃妥因等12种抗生素类药物对该病原菌敏感。Two dominant bacterial strains were isolated from the proboscis, muscle and liver of sick Babylonia areolata from a farm at Wengchang, Hainan. One of the stains was confirmed to be a bacterial pathogen orB. areolata by artificial infection and was determined to be Vibrio anguillarum Biovar [ Based on morphological detection and biochemical tests. The LDs0 of the pathogen to B. areolata was 1.7×10^6 cfu/g. Antibiotic sensitivity analysis indicated that the pathoge was sensitive to piperacillin, roxithromycin, cefobid, tobramycin, ceftriaxone, compound sulfamethoxazole,rifampicin, fortum and nitrofurantoin.

关 键 词：方斑东风螺(Babylonia areolata) 鳗弧菌生物变种Ⅰ(Vibrio ANGUILLARUM BIOVAR I) 分离 鉴定 药敏分析 

分 类 号：Q178.53[生物学—水生生物学]