α-酮戊二酸对脂多糖应激断奶仔猪空肠黏膜蛋白合成和抗氧化能力的影响  

Effects of α-ketoglutaric Acid on Protein Synthesis and Antioxidative Capacity in Jejunal Mucosa of Weaned Pigs Chronically Challenged with Lipopolysaccharide

作　　者：刘坚[1] 侯永清[1] 丁斌鹰[1] 刘玉兰[1] 朱惠玲[1] 王蕾[1] 李永塘[1] 

机构地区：[1]动物营养与饲料科学湖北省重点实验室,武汉工业学院,湖北武汉430023

出　　处：《中国畜牧杂志》2010年第11期35-38,共4页Chinese Journal of Animal Science

基　　金：国家自然科学基金项目(30871801);湖北省自然科学基金计划创新群体项目(2007ABC009)

摘　　要：试验研究日粮添加1%α-酮戊二酸(AKG)对多次脂多糖(LPS)刺激断奶仔猪空肠黏膜蛋白、RNA、DNA浓度和抗氧化能力的影响。选用24头健康杜洛克×长白×大白(7.25±0.23)kg断奶仔猪,随机分成3个处理,每个处理8个重复,公、母各半。对照组和LPS组饲喂基础日粮+1%淀粉、AKG组饲喂基础日粮+1%AKG,在试验第10、12、14、16天分别在LPS组和AKG组仔猪的腹膜注射80μg/kgBW脂多糖,对照组注射相应剂量的灭菌生理盐水。第17天清晨屠宰空腹仔猪,刮取空肠黏膜待测。结果表明:多次LPS刺激显著降低断奶仔猪空肠黏膜RNA/DNA、蛋白/DNA、蛋白质浓度及SOD活性(P<0.05),显著升高MDA含量(P<0.05),而日粮中添加AKG能在一定程度上抑制LPS刺激引起的空肠黏膜蛋白合成减少和抗氧化能力降低,维持肠道屏障完整性。This experiment was conducted to study the effects of α-ketoglutaric acid protein synthesis and antioxidative capacity in jejunal mucosa of weaned pigs chronically challenged with lipopolysaccharide. 24 healthy crossbred Duroc × （Landrace × Largewhite） weanling piglets （7.25 ± 0.23 kg BW） were randomly allocated to 3 treatments （control group, LPS control group and AKG group）. Each treatment included 8 replicates, 1 piglet per replicate, male and female in half. The control and LPS control group were fed the basal diet supplemented with 1% starch, while the AKG group was fed the basal diet supplemented with 1% α-ketoglutaric acid （AKG）. Piglets in the LPS control and AKG group were injected intraperitoneally with 80 μg/kg·BW lipopolysaccharide （LPS） every other day （on d 10, 12, 14 and 16）, whereas piglets in the control group were intraperitoneally injected with normal saline at the same dose. On d 17, all pigs were killed under anesthesia, the jejunal mucosa were collected. The resulted indicated that RNA/DNA, Protein/DNA, protein concentration and SOD activity of jejunal mucosa after LPS challenge were decreased （P 0.05）, and the MDA concentration was increased （P 0.05）, while dietary supplementation with 1%AKG could inhibit the decrease in protein synthesis and antioxidative capacity in jejunal mucosa induced by LPS challenge, AKG had protecting effects on intestinal barrier.

关 键 词：Α-酮戊二酸 脂多糖 空肠 黏膜蛋白 抗氧化 断奶仔猪 

分 类 号：S828.5[农业科学—畜牧学]