HPLC法测定防衰益寿丸中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量  

HPLC Determination of 2,3,5,4′- tetrahydroxystilbene-2 - O - β - D - glucoside in Fangshuaiyishou Pills

作　　者：孟蕾[1] 曹杰[2] 张海鸣[2] 沈圣民[2] 杜树山[2] 

机构地区：[1]北京师范大学校医院,北京100875 [2]中药资源保护与利用北京市重点实验室北京师范大学教育部资源药物工程研究中心,北京100875

出　　处：《辽宁中医杂志》2010年第6期1098-1099,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家科技支撑计划课题(2007BAI48B10);北京市新医药学科群重点支持项目(xk100270569)

摘　　要：目的:建立防衰益寿丸中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的高效液相含量测定方法。方法:色谱柱Diamonsil C18(4.6mm×150mm,5μm);以乙腈-水(18∶82)为流动相;流速:1mL·min-1;柱温:30℃;检测波长:320nm。结果:线性范围为0.055～0.165μg,精密度试验RSD为0.11%,回收率为96.40%,RSD为0.94%。结论:该方法快速、简便、准确、重现性较好,结果可靠,可为防衰益寿丸的质量评价提供有效手段。Objective： To estalish an accurate method for the determination of 2,3,5,4′-tetrahydroxystilbene -2-O-β-D-glucoside in Fangshuaiyishou Pills.Methods： Diamonsil C18（ 4.6mm × 150mm,5μm） column in an oven at 30℃ was need,with a mobile phase consisting of acetonitrile-H2O（ 18∶ 82） and a UV detector at 320 nm,the flow rate was 1.0mL·min -1.Results： The linear ranges of tetrahydropalmatine was 0.055 ～ 0.165μg.The RSD of measurement precision test was 0.11% ;The average recovery was 96.40% （ RSD = 0.94% ,n = 5） .Conclusion： The method was simple and accurate,and can be used for the quality control of Fangshuaiyishou Pills.

关 键 词：防衰益寿丸 2 3 5 4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]