猪孤雌胚胎体外序贯培养  

Sequential Culture of Porcine Parthenogenetic Embryos

作　　者：张立苹[1] 王亚刚[2,3] 李莉[2,3] 刘西梅 乔宪凤[2,3] 郑新民[2,3] 许生成[1] 

机构地区：[1]青海大学农牧学院,西宁810016 [2]湖北省农业科学院畜牧兽医研究所,武汉430064 [3]动物胚胎工程及分子育种湖北省重点实验室,武汉430064

出　　处：《中国畜牧兽医》2010年第6期47-50,共4页China Animal Husbandry & Veterinary Medicine

基　　金：转基因生物新品种培育重大专项(2008ZX08006-002;2008ZX08006-003;2008ZX08010-003;2008ZX0-8011-004);湖北省重点实验室(2010ZD123)

摘　　要：试验旨在探索不同培养体系和不同序贯培养法对猪孤雌胚胎体外发育的影响。采用普通培养和序贯培养2种培养方法进行猪卵母细胞的体外培养。结果表明,孤雌激活卵母细胞在NCSU23+0.4%BSA中分裂效果较好,而mTCM199+0.1%PVA则更有利于孤雌胚胎突破4细胞阻滞达到桑葚胚。在4种序贯培养中,NCSU23(0.4%BSA)+mTCM199(0.1%PVA)一组更适合于猪孤雌胚胎的体外培养。The purpose of the present study was to examine the effect of different sequential cultures on the porcine parthenogenetic embryos of development after activation.The ordinary culture and sequential culture were used in vitro culture of procine oocytes.The results showed that NCSU-23 supplemented 0.4% BSA was a better culture media for the oocytes development after activated in the early stage,but mTCM-199 supplemented 0.1% PVA facilitates parthenogenetic embryos breakthrough 4-cell block and reach morula;the sequential cultures system of NCSU-23 supplemented 0.4% BSA and mTCM-199 supplemented 0.1 % PVA could improve the development of porcine parthenogenetic embryos.

关 键 词：猪 孤雌胚 序贯培养 

分 类 号：S828[农业科学—畜牧学]