猪流感病毒的分离及NA基因的遗传进化分析  

Isolation of Swine Influenza A Viruses and Genetic Characterization for NA Genes of the Isolated Strains

作　　者：侯韶毅[1] 冯建远[1] 高永锐 卢桂娟 陆有飞 肖爱欢[1] 张民秀[1] 卢冰霞[1] 黄伟坚[1] 赵武[3] 

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]广西丽原生物股份有限公司,南宁530001 [3]广西兽医研究所,南宁530001

出　　处：《中国畜牧兽医》2010年第6期78-83,共6页China Animal Husbandry & Veterinary Medicine

摘　　要：通过鸡胚接种分离到3株猪流感病毒,经HI、RT-PCR鉴定,其中GXHZ株为H1N2亚型毒株,GXLZ、GXXY株为H3N2亚型毒株,并对分离株进行EID50测定及GXHZ株的猪体攻毒试验。所扩增分离到3株SIV病毒NA基因与A/Sw/Hainan/1/2005(H1N2)的核苷酸和氨基酸序列同源性最高。3株SIV分离株的NA基因在氨基端胞浆尾区均由K6→R6,在非极性跨膜区均由V20→M20。系统发育分析表明本试验所分离的3个SIV毒株与H1N2亚型毒株亲缘性最相近,且来源于猪源H1N2亚型流感病毒。从时间上也发现,3个分离株的NA基因与1996年以后获得的H3N2毒株亲缘关系很近,都隶属于一个亚分支。Three swine influenza virus strains were isolated from 9-11-day SPF chicken eggs in our study of these,GXHZ isolation was subtyped as H1N2 by HI assay and RT-PCR,and GXLZ,GXXY as H3N2.We detected the EID50 of these isolated strains and performed inoculation to pig with GXHZ isolation.NA genes amplificated from 3 isolated strains showed that their nucleotide and amino acid sequences had highest homology to A/Sw/Hainan/1/2005（H1N2）strain.Two substitution at 6（K6→R6）and 20（V20→M20）separately located on the amino acid cytoplasmic tail and nonpolar transmembrane domain of the NA molecules of isolated strains.Phylogenical analysis of the NA genes indicated that the NA genes of isolated strains were most closely related to H1N2 which derived from swine origin influenza virus.Moreover,three isolated strains were clustered with H3N2 strains isolated after 1996.

关 键 词：猪流感病毒 病毒分离 NA基因 遗传进化分析 

分 类 号：Q75[生物学—分子生物学]