益肾活血胶囊对高同型半胱氨酸血症兔血液ET、NO、NOS的影响  

Effect of Yishenhuoxue Capsule on NO,NOS and ET in Blood of Hyperhomocysteinemic Rabbits

作　　者：曲莹[1] 张继东[2] 

机构地区：[1]山东省职业卫生与职业病防治研究院,山东济南250002 [2]山东大学齐鲁医院,山东济南250012

出　　处：《预防医学论坛》2010年第6期481-482,489,共3页Preventive Medicine Tribune

基　　金：国家自然科学基金(30572452)

摘　　要：[目的]探讨益肾活血胶囊对高同型半胱氨酸(Hcy)血症兔血液内皮素(ET)、一氧化氮(NO)、一氧化氮合成酶(NOS)的影响。[方法]40只新西兰兔,随机平分为正常组、模型组、益肾活血胶囊小剂量组、益肾活血胶囊大剂量组及西药组(叶酸、维生素B6、维生素B12),除正常组外,其他组建立高Hcy血症及动脉粥样硬化(AS)动物模型,然后分别给予相应的药物治疗。高效液相色谱技术检测血浆Hcy浓度,放免法测定血浆ET浓度,硝酸还原酶法测定血清NO含量及总NOS活力。[结果]益肾活血胶囊大剂量组与模型组对照,血浆Hcy为(19.76±1.74)μmol/L、(32.12±5.29)μmol/L,血浆ET为(188.41±4.94)pg/ml、(280.41±17.490)pg/ml,血清NOS为(10.90±0.71)U/ml、(5.32±0.96)U/ml,血清NO为(126.39±15.99)μmol/L、(55.81±4.95)μmol/L)。[结论]益肾活血胶囊可明显降低血浆Hcy水平,抑制Hcy诱导的动脉粥样硬化形成,其机制可能与减轻血管内皮功能损伤有关。[Objective] To explore the effect of Yishenhuoxue Capsule on NO,NOS and ET in blood of hyperhomocysteinemic rabbits.[Methods]Forty male New Zealand rabbits were divided into five groups：control,model,low dose YC,high dose YC and western medicine（folic acid,Vitamin B6 and Vitamin B12）.Apart from the control group,the rabbits in other groups underwent balloon catheter injury and high methionine diet to induce hyperhomocysteinemia and atherosclerosis,followed by treatment with different drugs.The results were observed and evaluated.[Results]Compare high dose YC group with model group,plasma Hcy（19.76±1.74,32.12±5.29 μmol/L）,plasma ET（188.41±4.94,280.41±17.49 pg/ml）,serum NOS（10.90±0.71,5.32±0.96 U/ml）,serum NO（126.39±15.99,55.81±4.95μmol /L）.[Conclusion]YC decreases plasma Hcy level and subsequently reverses the aortic lesion induced by Hcy.The mechanism may be related to its suppressive effect on vas endothelial lesion.

关 键 词：益肾活血胶囊 同型半胱氨酸 内皮素 一氧化氮 一氧化氮合成酶 

分 类 号：R96[医药卫生—药理学]