体外氰戊菊酯暴露抑制小鼠海马神经元突起形成  

Fenvalerate inhibits the neurite growth of hippocampus in vitro

作　　者：杨叶新[1] 王取南[1] 夏玉宝[1] 包训迪[1] 柴小玉[1] 李芳芳[1] 张颖平[2] 

机构地区：[1]安徽医科大学卫生毒理学系,合肥230032 [2]安徽医科大学第一附属医院神经外科,合肥230022

出　　处：《安徽医科大学学报》2010年第3期289-293,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金项目(编号:30571585);安徽省自然基金项目(编号:050430802)

摘　　要：目的探讨氰戊菊酯对小鼠海马神经元突起的影响。方法取孕18d的ICR小鼠胚胎海马细胞体外培养,建立小鼠海马神经元和星形胶质细胞混合培养体外模型。以1、5、10、50mg/L的氰戊菊酯对培养8d后的细胞进行染毒,乳酸脱氢酶(LDH)比色法测定染毒48h后的细胞毒性;采用荧光免疫细胞化学的方法研究小鼠海马神经元和星形胶质细胞的相对含量及海马神经元突起状况。结果在对海马神经细胞不产生明显细胞毒性的前提下,氰戊菊酯可以引起神经元缺失,而且随着剂量的增加,神经元突起的数目及长度均随之明显下降。结论氰戊菊酯可能特异损害和(或)Objective To investigate the effects of fenvalerate on the hippocampus neurons of mouse in vitro.Methods The cells were derived from 18 day embryonic mouse hippocampus.Dose-dependent cytotoxicity of fenvalerate on the hippocampus cells at 48 h was deceted by LDH-releasing assay.The immunocytochemistry staining was used to determine the quantities of neurons and astroglias,and the neurite growth of hippocampus. Results Neurons was more vulnerable to fenvalerate than astroglias and fenvalerate decreased the length and number of neurite in a dose-dependent manner.Conclusion Fenvalerate can disrupt and（or） inhibit neurite growth in neurodevelopment,which may induce developmental neurotoxicity.

关 键 词：除虫菊酯类/药理学 神经元/药物作用 海马/药物作用 

分 类 号：R979.9[医药卫生—药品] R322.8[医药卫生—药学]