以CDC2激酶为靶点的反义基因治疗大鼠慢性增生性胆管炎  

Anti-proliferative effect of CDC2 kinase antisense on proliferative cholangitis

作　　者：周涌[1] 李富宇[1] 程南生[1] 蒋力生[1] 李宁[1] 李全生[1] 

机构地区：[1]四川大学华西医院肝胆外科,成都610041

出　　处：《中华普通外科杂志》2010年第5期398-400,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金（30801111）和教育部博士点新教师基金（200806101065）资助项目

摘　　要：目的 胆结石的术后高复发率和胆道再狭窄率均与术后遗留的慢性增生性胆管炎（proliferative cholangitis,PC）密切相关,故探讨CDC2 kinase shRNA对PC的抗增殖疗效和抑制成石潜力的可行性意义重大.方法 向大鼠PC模型的胆总管内注入0.5 ml的CDC2 kinase shRNA.数据采用方差分析、q检验.结果 CDC2 kinase shRNA可通过抑制CDC2 kinase,PCNA和Procollagen Ⅰ的表达,有效遏制胆道黏膜上皮、黏膜下腺体和胆管壁胶原纤维的过度增殖,并使内源性β-葡萄糖醛酸酶的分泌减少50%,从而降低PC的成石潜力.结论 CDC2 kinase shRNA对PC的抗增殖疗效可能有助于预防肝内胆管结石的术后复发和胆道再狭窄.Objective Proliferative cholangitis （PC） is responsible for stone recurrence and biliary restenosis, this study was to investigate the and-proliferative effect of CDC2 kinase shRNA on PC. Methods The common bile duct of PC rat model was given an intralumenal administration of 0. 5 ml of CDC2 kinase shRNA. Results CDC2 kinase shRNA treatment effectively inhibited the expression of CDC2 kinase,PCNA, and procollagen I , resulting in the inhibition of hyperplasia of biliary epithelium, submucosal gland, and collagen fibers. Also, the lithogenic potentiality of PC decreased due to the inhibition of endogenous β-glucuronidase secretion. Conclusion The anti-proliferative effect of CDC2 kinase shRNA on PC may prevent biliary restenosis and stone recurrence.

关 键 词：胆结石 胆管炎 CDC2蛋白激酶 

分 类 号：R657.4[医药卫生—外科学]