金属蛋白酶1组织抑制因子在大鼠自体移植静脉中的表达研究  

TIMP-1 inhibitor expression in autologous vein graft

作　　者：张绍坤[1] 郭冰[1] 周玉峰[1] 宁均[1] 李春来[1] 王雷[1] 

机构地区：[1]牡丹江市第一人民医院头颈科,黑龙江省157011

出　　处：《中华普通外科杂志》2010年第5期405-408,共4页Chinese Journal of General Surgery

摘　　要：目的 研究金属蛋白酶1组织抑制因子（tissue inhibitor of metalloproteinase-1,TIMP-1）在大鼠自体移植静脉血管平滑肌细胞中的表达和动态变化的意义.方法 建立大鼠自体血管移植模型,移植后不同时间分别切取移植静脉,应用HE染色、免疫组织化学和原位杂交方法动态观察TIMP-1的表达和变化情况.结果 移植血管组织病理学改变：血管移植后1周可见内膜增生（intimal hyperplasia：IH）,2～4周达到高峰.原位杂交结果：TIMP-1mRNA在移植后24 h出现细胞阳性表达,72 h阳性表达明显增多,1-2周时表达达到高峰,与移植后1 d相比,差异有统计学意义（P〈0.01）.免疫组织化学染色：静脉移植后72 h出现TIMP-1的表达,1周时增加明显,2周时表达最强,与移植后1 d相比,差异有统计学意义（P〈0.01）.结论 在自体移植静脉中存在TIMP-1的激活.自体静脉移植于动脉后,内源性TIMP-1的分泌增加不足以抑制内膜增生.Objective To investigate the expression of matrix metalloproteinases 1 （TIMP-1）products inhibitor on smooth muscle cell （SMC） proliferation in rat autologous vein graft.Methods Autogenous vein transplantation model was established in 30 Wistar rats. The vein grafts were harvested at different time after grafting. The change of TIMP-1 was detected by using hematoxylin and eosin, immunohistochemistry and in situ hybridization （ISH）. Results 1. Changes of histopathology in vein grafts： Intimal hyperplasia （IH） could be seen in 7 - 14 days, the peak at 2 - 4 weeks after operation.2. ISH results： TIMP-1mRNA positive cells appeared at 24 hours, increased significantly in 72 hours and reached the peak at 1 - 2 week after operation. There was significant difference between day 1 and 2 week.TIMP-I expression was not detected in normal vessels （P 〈0. 01）. 3. Immunohistochemistry results： There was trace TIMP-I expression in normal vessels. The expression of TIMP-1 appeared at 72 hours after vein graft, increased mostly in 1 week, reached the peek at 2 week and reduced later. There was significant difference between day 1 and 2 week （P 〈 0. 01）. Conclusions 1. The activation of TIMP-1 exists in autogenous vein grafts. 2. The intima experienced hyperplasia in spite of increased secretion of endogenous TIMP-1 after autogenous vein grafts.

关 键 词：移植 金属蛋白酶1组织抑制剂 内膜增生 静脉移植 

分 类 号：R654.4[医药卫生—外科学]