人类白细胞抗原G基因14bp缺失多态性与重度子痫前期发病的关系  

Human leukocyte antigen-G 14 bp deletion polymorphism in severe pre.eclampsia

作　　者：张展[1] 王婧彦[1] 张琳琳[2] 郭上[3] 贾莉婷[2] 李辉[2] 李静[1] 琚娟[1] 荣守华[2] 

机构地区：[1]郑州大学第三附属医院妇产科,450052 [2]郑州大学第三附属医院检验科,450052 [3]上海交通大学临床医学系

出　　处：《中华妇产科杂志》2010年第5期348-352,共5页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金（30872316）

摘　　要：目的 探讨人类白细胞抗原G（HLA-G）基因第8外显子14 bp缺失多态性与重度子痫前期发病的关系.方法 选择2008年10月至2009年2月在郑州大学第三附属医院妇产科住院的42例孕晚期重度子痫前期孕妇及其新生儿为重度子痫前期组.另选择同期正常孕晚期孕妇及其新生儿45例为健康晚孕组,两组孕妇均为汉族居民.采用PCR技术对两组孕妇及其新生儿进行HLA-G基因第8外显子14 bp缺失多态性的等位基因分析,分别比较两组孕妇及其新生儿之间等位基因及基因型的频率分布,通过母、儿基因型配伍,比较两组间基因型配伍频率分布的差异.结果 （1）重度子痫前期组中母、儿均为14 bp缺失纯合子（-14 bp/-14 bp）的基因型配伍的频率为14%（6/42）,显著低于健康晚孕组的33%（15/45）,两组比较,差异有统计学意义（P=0.038）;（2）重度子痫前期组孕妇HIA-G第8外显子14 bp缺失多态性的等位基因频率、基因型频率与健康晚孕组比较,差异均尤统计学意义（P〉0.05）;（3）重度子痫前期组新生儿HLA-G第8外显子14 bp缺失多态性等位基因＋14 bp频率为44%（37/84）、-14 bp为56%（47/84）,健康晚孕组新生儿＋14 bp为30%（27/90）、-14 bp为70%（63/90）,两组比较,差异虽无统计学意义,但存在差异性趋势（P=0.055）;重度子痫前期组新生儿（-14 bp/-14 bp）基因型频率为29%（12/42）,与健康晚孕组的49%（22/45）相比,存在差异性趋势（P=0.052）.结论 中国汉族孕妇中,HIJA-G第8外显子14 bp缺失多态性与重度子痫前期的发病有关;母、儿均为缺失纯合子（-14 bp/-14 bp）基因型配伍者,发生重度子痫前期的风险会降低.Objective To investigate the relationship between human leukocyte antigen -G（HLA-G） gene Exon 8 14 bp deletion polymorphism and the pathogenesis of severe pre-eclampsia. Methods Forty-two pregnant women with severe pre-eclampsia, who admitted to the Third Affiliated Hospital of Zhengzhou University from October 2008 to February 2009, and their newborns were chosen as the severe pre-eclampsia group. Another 45 healthy gravidas at the third trimester and their newborns were chosen as the control. All gravidas in both groups were Han Nationality. HLA-G Exon 8 genotyping was detected by PCR in both groups and the allele frequencies and genotype frequencies were compared between the two groups. The genotype frequencies of maternal-neonatal pairs were also analyzed. Results （1） In the severe pre- eclampsia group, 14% of the maternal-neonatal pairs were homozygote of 14 bp deletion, and significantly higher frequency 33% （15/45） was found in the control group （P =0. 038）. （2） No significant difference was found in the allele frequencies and genotype frequencies of HLA-G 14 bp deletion polymorphism among all the mothers between the two groups （ P 〉 0. 05 ）. （3） The ＋ 14 bp and - 14 bp allele frequencies of HLA-G 14 bp deletion polymorlahism in newborns in the severe ore-eclamosia croup were 44% （37/84） and 56% （47/84）, respectively, and 30% （27/90） and 70% （63/90） in the control group. Although there was no significant difference between the two groups, but differences in trends was identified （ χ^2 = 3. 678 P = 0. 055 ） ; The genotype （ - 14 bp/- 14 bp） frequency of neonates in the severe pre-eclampsia group showed no difference compared with that in the control group E 29% （12/42） vs 49% （22/45） ] , but differences in trends was also found （P =0. 052）. Conclusions HLA-G 14 bp deletion polymorphism is associated with the susceptibility of severe pre-eclampsia in Chinese Han nationality. Maternal-fetal genotype pairs of - 14 bp/- 14 bp may have reduced

关 键 词：先兆子痫 HLA抗原 组织相容性抗原I类 多态现象 遗传 序列缺失 

分 类 号：R714.245[医药卫生—妇产科学]