氯化锰对鸡支持-生精细胞凋亡及Bak和Bcl-x基因表达的影响  

Effect of MnCl2 exposure on apoptosis of cock sertoli-germ cell and on expression of Bak and Bcl-x mRNAs

作　　者：毕明玉[1] 张子威[1] 陈蕾[1] 唐洪鹏[1] 徐世文[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医科学》2010年第5期518-522,共5页Chinese Veterinary Science

摘　　要：为探讨氯化锰(MnCl2)对鸡支持-生精细胞凋亡及Bak、Bcl-x基因mRNA表达的影响,取对数生长期鸡支持-生精细胞,用含0、2、3、4mmol/L MnCl2的培养液培养24h,采用原位末端脱氧核苷酸转移酶标记法(TUNEL)检测鸡支持-生精细胞凋亡,采用实时定量PCR检测Bak、Bcl-x基因mRNA的表达量。结果显示,MnCl2处理组细胞与对照组相比,鸡支持-生精细胞凋亡指数逐渐升高(P<0.01);Bak基因的表达量上调(P<0.01),Bcl-x基因的表达量下降(P<0.01)。说明MnCl2可通过改变Bak、Bcl-x基因mRNA表达量导致鸡支持-生精细胞发生凋亡。To determine the effect of MnCl2 exposure on apoptosis of cock sertoli-germ cell and on expression of Bak and Bcl-x mRNAs,cock sertoli-germ cells were cultivated in DMEM with the final concentrations of 0,2,3 and 4mmol/L MnCl2 respectively for 24h.The apoptosis was determined by TUNEL assay,and the expression amounts of Bak and Bcl-x mRNAs were examined by real-time fluorescence quantitative PCR.In result,compared with control group,the apoptosis index（AI） of cock sertoli-germ cell significantly increased（P0.01）,the expression of Bak mRNA increased（P〈0.01） while the expression of Bcl-x mRNA decreased（P〈0.01） in 2,3 and 4mmol/L MnCl2 treatment groups.The result indicated that MnCl2 was able to induce apoptosis of cock sertoli-germ cell by up-regulating the expression of Bak mRNA and down-regulating the expression of Bcl-x mRNA.

关 键 词：锰 鸡支持-生精细胞 细胞凋亡 BAK基因 BCL-X基因 

分 类 号：S856.9[农业科学—临床兽医学]