己酮可可碱对瘢痕疙瘩成纤维细胞增殖的影响  

Effect of pentoxifylline on proliferation of human keloid fibroblasts

作　　者：冯红霞[1] 辛燕[1] 郝玉琴[1] 康春义[1] 

机构地区：[1]内蒙古医学院第三附属医院-包钢医院皮肤科,内蒙古自治区包头市014010

出　　处：《中国组织工程研究与临床康复》2010年第11期1923-1926,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:近年来发现己酮可可碱有广泛的抗纤维化作用,但己酮可可碱对人瘢痕疙瘩成纤维细胞增殖的抑制作用却少见报道,且其最大抑制剂量尚无定论。目的:观察己酮可可碱对瘢痕疙瘩成纤维细胞增殖的作用,并筛选己酮可可碱的最大抑制浓度。方法:以人瘢痕疙瘩组织作成纤维细胞原代培养,传至第5～8代后将细胞分为实验组和对照组,实验组加入质量浓度为0.1,0.25,0.5,1.0,2.0,3.0g/L的己酮可可碱,利用MTT比色法检测成纤维细胞的增殖活性。结果与结论:与对照组相比,实验组成纤维细胞增殖抑制率较高(P<0.05),质量浓度在0.1～2.0g/L范围内呈明显的量效、时效关系,96h处于较高水平。实验组最大抑制率为53.37%,最大抑制质量浓度为2.0g/L。结果表明己酮可可碱对瘢痕疙瘩成纤维细胞的增殖有明显的抑制作用,最适抑制质量浓度为2.0g/L,发挥抑制作用最强的时间为给药后96h。BACKGROUND： In recent years, pentoxifylline has been found to have a wide range of anti-fibrosis capacity. However, there are few studies explore the suppress effect of pentoxifylline on fibroblasts in human keloid, and the maximum inhibitory concentration remains poorly understood. OBJECTIVE： To study the effect of pentoxifylline on proliferation activity of human keloid fibroblasts and to select the maximum inhibitory concentration. METHODS： Human keloid fibroblasts were used as original cells, passaged till the 5th to the 8th generations, and then divided into the experimental and control groups. Pentoxifylline with concentrations of 0.1, 0.25, 0.5, 1.0, 2.0 and 3.0 g/L were added to the experimental group. The effects of different concentrations of pentoxifylline on proliferation of keloid fibroblasts were detected by MTT chromometry. RESULT AND CONCLUSION： Compared with the control group, the inhibitory effect of pentoxifylline on the proliferation of keloid fibroblasts was more evident in the experimental group （P 0.05）. The inhibition rates of pentoxifylline on proliferation of keloid fibroblasts showed apparently time-and dose-effect relationships within the concentration of 0.1-2.0 g/L, which presented a greatest level at 96 hours after culture. The maximum inhibitory rate was 53.37%, and the concentration was 2.0 g/L in the experimental group. Consequently, pentoxifylline plays a notable inhibitory role in the proliferation of human keloid fibroblasts with concentration of 2.0 g/L at 96 hours after culture.

关 键 词：己酮可可碱 瘢痕疙瘩 成纤维细胞 细胞培养 细胞增殖 皮肤组织工程 

分 类 号：R318[医药卫生—生物医学工程]