力学载荷下大鼠激素性股骨头坏死的改变  

Changes of glucocorticoid-induced osteonecrosis of the femoral head under mechanics load

作　　者：王志钢[1] 马信龙[1] 

机构地区：[1]天津医科大学总医院骨科,天津市300052

出　　处：《中国组织工程研究与临床康复》2010年第11期1963-1966,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:力学载荷下大鼠激素性股骨头坏死表现更为典型。目的:建立大鼠激素性股骨头坏死正常负重和超负重模型,对使用激素后不同时段大鼠股骨头进行观察,探究在力学载荷下大鼠激素性股骨头的相关改变。方法:4月龄健康Wistar大鼠随机分成实验组和对照组。两组大鼠均臀肌注射地塞米松磷酸钠(20mg/kg),1次/周,共计8周。实验组,置于1km/h跑步机中,强迫其跑动,形成股骨头坏死超负重模型;对照组,生理状态下正常负重。分别于2,4,6,8周处死大鼠,取右侧股骨头标本,于EnduraTEC ELF3200生物力学材料动态力学性能测试系统行压力测定,计算样本纵向最大位移、刚度吸收等生物力学参数,计算空骨陷窝数,并行免疫组织化学法Bcl-2染色,比较不同组之间的累计吸光度值。结果与结论:实验组8周时病理学呈股骨头坏死表现,各个时期股骨头骨小梁宽度、刚度均低于同期对照组;最大形变均高于同期对照组(P<0.05)。实验组Bcl-2累计吸光度与对照组比较,第4周起差异有显著性意义(P<0.05),实验组Bcl-2表达随激素注射时间增加有显著性意义(P<0.05)。结果提示,在激素性股骨头坏死中力学载荷使骨细胞凋亡明显,软骨细胞坏死修复增加,并增多骨小梁断裂,不利于保护骨小梁的完整性,成为引起激素性股骨头坏死塌陷的直接外部因素,并出现更显著的股骨头坏死表现。BACKGROUND： Femoral head necrosis induced by glucocorticoid is typical under mechanics load. OBJECTIVE： To create the rat models of the femoral head necrosis by long-term glucocorticoid treatment, and to observe the changes of femoral head necrosis induced by glucocorticoid under mechanics load. METHODS： Wistar rats with 4-month old were randomly divided into the experimental and control groups. After the buttocks injection of dexamethasone 20mg/kg weakly for 8 weeks, rats in the experimental group were made to run on the running machine at a speed of 1 km/h to forme the hyper-weight model; at the same time, those in the control group were with the normal physiological weight. Rats in both groups were sacrificed at weeks 2, 4, 6 and 8. The left legs were taken for the biomechanics test on the EnduraTEC ELF3200, the biggest vertical deformation and stiffness were calculated, the number of empty osteocyte lacunas was calculated, and the cumulative absorbance value was compared between two groups. RESULTS AND CONCLUSION： The femoral head presented with necrosis at 8 weeks in the experimental group, and the width of bone trabecula, and the stiffness of which were smaller than that of the control group, but the biggest vertical deformation was greater the concurrent control group （P 0.05）. The Bcl-2 cumulative absorbance value of 2 groups had significant differences at 4 weeks （P 0.05）, which was increased with time prolonged in the experimental group （P 0.05）. The results demonstrated that： in glucocorticoid-induced osteonecrosis of the femoral head, mechanics load induces the obviously osteocyte necrosis, more cartilage repair, and trabeculae rupture, which is considered as the direct reason of the necrosis of the femoral head. The typical femoral head necrosis is presented.

关 键 词：股骨头坏死 力学载荷 激素 骨组织工程 生物力学 

分 类 号：R318[医药卫生—生物医学工程]