人胚胎发育时期足部组织中锌指蛋白548可能和HOXD13相互作用形成蛋白复合体而发挥作用吗?  

May HOXD13-zinc finger protein 548 complex develop in foot tissue during human embryonic development?

作　　者：王莉莉[1] 辛娜[2] 孙开来[2] 

机构地区：[1]中国医科大学附属盛京医院实验研究中心,辽宁省沈阳市110004 [2]中国医科大学基础医学院医学遗传学教研室,辽宁省沈阳市110001

出　　处：《中国组织工程研究与临床康复》2010年第11期1992-1994,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家重点基础研究发展规划资助项目(2001CB510301)~~

摘　　要：背景:HOX基因家族是一个高度保守的转录因子家族,该家族在胚胎发育阶段基本体轴和次级体轴的形成中起作用,同时在中枢神经系统、中轴骨、胃肠和泌尿生殖系及外生殖器和肢体的发育中起重要作用。目的:观察孕14周人胚胎足部发育过程中与HOXD13相互作用的蛋白质。方法:取胎儿足部组织,应用蛋白提取试剂盒提取蛋白,加入6μgHOXD13抗体进行免疫沉淀实验获取HOXD13相互作用的蛋白质,以加入非特异的IgG作为阴性对照。考马斯亮蓝染色后观察沉淀下来蛋白质形成的条带,切取需要条带送质谱分析。结果与结论:利用HOXD13抗体在孕14周人胚足部组织蛋白质中获取了一个可能与HOXD13相互作用的蛋白质,相对分子质量约为60000,经质谱鉴定后为锌指蛋白548。在孕14周人胚足部发育过程中,HOXD13可能和锌指蛋白548结合形成蛋白复合体而发挥转录调控作用。BACKGROUND： The HOX gene family is a highly conserved transcription factor family, which affects the formation of basic axis and secondary axis during embryonic development, at the same time, it plays a pivotal role in the development of the central nervous system, axial skeleton, stomach intestine, urogenital system and external genitalia. OBJECTIVE： To investigate the interactional protein of HOXD13 in human embryo foot development at 14 weeks pregnancy. METHODS： Fetal foot tissues were harvested and protein extraction was performed using protein extraction kit, 6 μg HOXD13 antibody was added for immunoprecipitation experiment, which aims to obtain HOXD13 interactional proteins, while the non-specific IgG served as a negative control. Subsequent to Bradford staining, the sediment protein bands were cut for mass spectrometry. RESULTS AND CONCLUSION： We obtained an interactional protein of HOXD13 in the human embryo foot at 14 weeks pregnancy, at the relative molecular mass of approximately 60 000. The protein was identified as zinc finger protein 548 using mass spectrum. HOXD13 may play a very important role in transcriptional control by HOXD13-zinc finger protein 548 protein complex in the human embryo foot at 14 weeks pregnancy.

关 键 词：HOXD13 相互作用蛋白 ZNF548 转录调控 组织工程 

分 类 号：R318[医药卫生—生物医学工程]