AhNCED1基因转化花生研究  

Transformation AhNCED1 Gene in Peanut(Arachis hypogaea L.)

作　　者：覃铭[1,2] 胡博[1] 刘璨[1] 李玲[1] 罗虹[1] 

机构地区：[1]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州510631 [2]北京师范大学珠海分校工程技术学院,广东珠海519085

出　　处：《热带亚热带植物学报》2010年第3期277-282,共6页Journal of Tropical and Subtropical Botany

基　　金：广东省科技计划项目(20065030014);广东省自然科学基金(06025049);广东省教育厅自然科学重点项目(C10033);国家自然科学基金面上项目(30771297)资助

摘　　要：构建转化AhNCED1基因花生(Arachis hypogaea L.)过表达载体35S∷AhNCED1∷GUS,用OD600=0.8的LBA4404农杆菌液浸染汕油523,抗性芽诱导率达100%。PCR检测89株筛选苗,43株呈阳性,GUS检测阳性率为50%。转基因植株地上部分ABA含量增加;PEG胁迫10 h,转基因植株叶片AhNCED1蛋白表达增强,内源ABA水平积累,超氧化物水平降低。9-cis-epoxycarotenoid dioxygenase（NCED） is the key enzyme of ABA biosynthesis in higher plant.The expression vector 35S∷AhNCED1∷GUS for peanut（Arachis hypogaea L.） was constructed.Then AhNCED1 gene was transformed into cotyledon of ＇Shanyou 523＇,which is sensitive to drought stress,by immersing into Agrobacterium tumefciens strain LBA4404 bacterial suspension solution.The induction rate of adventitious buds with kanamycin-resistant was 100%,and in which there were 48.3% and 50% positive plantlets by PCR amplification and GUS histochemical identification,respectively.The ABA contents in aboveground organs of transgenic plants were higher than that in the wild（non-transformation） plants.When the leaves of the transgenic AhNCED1 plants subjected to PEG stress for 10 h,the expressions of AhNCED1 protein and endogenous ABA content were increased,as well as superoxide level decreased.

关 键 词：AhNCED1基因 过表达 转化 花生 ABA 

分 类 号：Q785[生物学—分子生物学]