构建球磨碳酸钙/聚磷酸钙纤维/聚乳酸组织工程支架复合材料  

Construction of CaCO_3/calcium polyphosphate/poly-(L-Lactic acid) composite tissue engineered scaffolds

作　　者：朱凌云[1] 王彦平[1] 石宗利[1] 张红梅[1] 

机构地区：[1]兰州交通大学机电工程学院,甘肃省兰州市730070

出　　处：《中国组织工程研究与临床康复》2010年第21期3823-3826,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：甘肃省自然科学基金项目(096RJZA085)资助~~

摘　　要：背景:近年来国内外在骨与软骨组织支架材料方面取得了积极的成果,但仍存在炎症反应高、生物相容性低等许多问题,因此复合材料技术是解决目前存在问题的重要途径之一。目的:制备球磨碳酸钙/聚磷酸钙纤维/聚乳酸组织工程支架复合材料。方法:采用溶媒浇铸、粒子滤取技术与气体发泡相结合的方法制备出球磨碳酸钙/聚磷酸钙纤维/聚乳酸组织工程支架复合材料。测试该支架复合材料的物理、力学及降解性能,并用扫描电子显微镜对其微观结构进行观察。结果与结论:制备出孔隙率在60%～80%之间的球磨碳酸钙/聚磷酸钙纤维/聚乳酸组织工程支架复合材料,具有三维、连通、微孔网状结构,其密度的实验值和计算值基本相吻和;压缩模量值均在3MPa以上,能够满足软骨组织工程支架复合材料对压缩模量的要求。随着降解时间的延长,支架复合材料的降解速率在增大;球磨碳酸钙/聚磷酸钙纤维/聚乳酸组织工程支架复合材料的降解液的pH值基本保持在6～7.5之间,球磨碳酸钙粉末的加入稳定了降解液的pH值。该支架复合材料降解一定时期后仍为三维、连通、微孔网状结构。BACKGROUND：There are some problems such as high inflammatory reaction and low biocompatibility although some progressive achievements have been obtained in the research of bone and cartilage tissue engineering.Technology of composite scaffold is an important method to dissolve the problems.OBJECTIVE：To fabricate CaCO3 /calcium polyphosphate /poly-（L-Lactic acid）（CaCO3/CPP/PLLA） composite tissue engineered scaffolds.METHODS：The ball milling CaCO3/CPP/PLLA composite tissue engineered scaffolds were fabricated with a solvent-casting,particulate-leaching and gas foaming methods.The properties of physical mechanics and degradation rates in vitro were tested.The microstructure of cross section of scaffold composites was observed by scanning electron microscope.RRSULTS AND CONCLUSION：The ball milling CaCO3/CPP/PLLA composite tissue engineered scaffolds were fabricated with the porosity of scaffolds varying 60% and 80%,which has three dimensional,interconnect,micro-hole network structure.Its density of experimental data was basically coincident with its calculated data.The compressive modulus was more than 3 MPa and it met the requirements of composite tissue engineered scaffolds.The degrade rate was increased with the prolonging of the degradable times.The degradable liquid pH value was maintained between 6 and 7.5.The degradable liquid pH value was stabled with the addition of the powder ball milling CaCO3.The microstructure of scaffold composites still has three dimensional interconnect micro-hole network structure after degrading.

关 键 词：支架 组织工程 复合材料 降解 组织工程支架材料 

分 类 号：R318[医药卫生—生物医学工程]