壳聚糖-肝素静电自组装多层膜在钛表面进行的生物化修饰  

Titanium surface modification with chitosan-heparin multilayers using electrostatic self-assebly technique

作　　者：舒瑶[1] 李全利[2] 欧国敏[3] 宫苹[3] 邹敬才[1] 

机构地区：[1]解放军军事医学科学院附属医院口腔科,北京市100070 [2]安徽医科大学口腔医学院,安徽省合肥市230032 [3]四川大学华西口腔医学院,四川省成都市610041

出　　处：《中国组织工程研究与临床康复》2010年第21期3832-3838,共7页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：安徽省高等学校自然科学基金重点项目(KJ2009A169);安徽省科技攻关项目(08010302196)~~

摘　　要：背景:基于聚电解质阴阳离子交替组装的静电自组装技术可在温和、简单、易控的条件下实现多种生物大分子在材料表面的固定,已成为生物材料表面设计的重要手段。目的:利用静电自组装技术将具有生物活性的壳聚糖和肝素固定在钛表面,实现钛表面的氨基多糖生物化修饰,构建一种钛种植体材料的新型生物化表面,以改善钛的细胞相容性。方法:首先采用NaOH处理钛基材,获得多孔、负电荷的钛表面;然后吸附一层正电荷的聚赖氨酸;最后,多次交替吸附负电荷的肝素和正电荷的壳聚糖,形成以壳聚糖为最外层的多层膜结构。通过漫反射红外光谱扫描电镜和原子力显微镜对多层膜进行表征。并与成骨细胞共培养,观察成骨细胞的黏附、增殖以及分化情况。结果与结论:红外光谱、原子力显微镜、扫描电镜结果表明肝素-壳聚糖多层膜逐渐形成。此涂层可促进成骨细胞的黏附、增殖和分化。肝素-壳聚糖多层膜有望成为一种新型的生物化钛表面,从而改善钛表面的生物相容性。BACKGROUND：Based on the consecutive adsorption of polyanions and polycations via electrostatic interactions,electrostatic self-assebly（ESA） technique has emerged as a versatile,inexpensive yet efficient technique to ＂build＂ biologically active surfaces for modification of biomaterial surface.OBJECTIVE：Bioactive chitosan and heparin multilayers fixed on titanium surface via ESA technique are expected to be a new biologic surface to improve the cell biocompatibility of titanium.METHODS：The film growth was initialized by depositing one layer of positively charged poly-L-lysine（PLL） on the NaOH-treated titanium substrate（negatively charged surface）.Then,the film was formed by the alternate deposition of negatively charged heparin（Hep） and positively charged chitosan（Chi） via electrostatic interactions of polyelectrolytes,and terminated with an outermost layer of Chi.The chemical composition,surface topography as well as roughness were investigated by using diffuse reflectance Fourier transform infrared spectroscopy（DR-FTIR）,scanning electric microscope（SEM） and atomic force microscope（AFM）,respectively.We cultured osteoblasts on the surface of modified-titanium（Ti/Chi） and pure Ti（cpTi） respectively in vitro to observe morphologic change of cell attachment and spreading by SEM,and to estimate cell proliferation and differentiation by MTT test and ALP activity analysis.RESULTS AND CONCLUSION：The analysis of DR-FTIR,SEM,and AFM confirmed that Chi-Hep multilayers formed gradually on the titanium surface.The film could improve the adherence,proliferation and differentiation of osteoblast.These results may provide a basis for the preparation of titanium surface modification for dental implants.

关 键 词：静电自组装 钛 表面改性 壳聚糖 肝素 

分 类 号：R318[医药卫生—生物医学工程]