青壮年猝死综合征心肌细胞内Cx43的表达  

Expression of connexin 43 in cardiomyocytes of sudden manhood death syndrome

作　　者：罗斌[1] 程灵犀[1,2] 林俊莲[2] 刘水平[1] 唐双柏[1] 孔小平[2] 张能培 韩奇杰[4] 汪冠三[2] 许心舒[2] 石河 

机构地区：[1]中山大学中山医学院法医学系,广东广州510080 [2]广州市刑事科学技术研究所,广东广州510030 [3]东莞市刑科所,广东东莞523000 [4]深圳市公安局坪山分局,广东深圳518118

出　　处：《中国法医学杂志》2010年第3期159-161,共3页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(39700167)

摘　　要：目的观察连接蛋白43(Cx43)在青壮年猝死综合征(SMDS)者心肌细胞的表达,并探讨其法医学意义。方法筛选法医尸检案例45例,其中SMDS组、冠心病猝死组及对照组各15例。采用免疫组织化学和图像分析技术对不同组别Cx43蛋白在心肌细胞内的表达进行阳性单位(PU)定量检测,分析Cx43蛋白在各组表达的差异。所得数据进行统计学分析。结果 SMDS组心肌Cx43染色明显减弱,阳性着色条带分布不均、深浅不一,有的呈散在颗粒状;冠心病猝死组亦见类似变化;对照组未见明显变化。经统计分析发现,3组心肌细胞内Cx43表达的PU值存在显著性差异(P<0.05)。结论 SMDS死前存在心肌缺血及心电紊乱,应属心性猝死范畴。Objective To study the expression of connexin43 （Cx43） in cardiomyocytes in cases of sudden manhood death syndrome（SMDS） and explore its medicolegal significance. Methods Obtained 45 autopsy cases from 3 different groups （SMDS group,coronary atherosclerotic heart disease group and control group）. Cx43 protein in myocardium was detected by immunohistochemical techniques. Computerized image analysis was employed to measure the Cx43 positive units（PU） of cardiomyocytes,the data were analyzed statistically. Results Expression of Cx43 in myocardium decreased obviously in SMDS group. Similar changes were also observed in coronary atherosclerotic heart disease group but no obvious changes in the control group. There were significant differences of Cx43 positive units in the myocardium among the three groups （P0.05）. Conclusions Immunohistochemical detection of Cx43 in myocardium may effectively help postmortem diagnosis of SMDS.

关 键 词：法医病理学 连接蛋白43 青壮年猝死综合征 免疫组织化学 

分 类 号：D919[医药卫生—法医学]