氟乙酰胺代谢产物氟离子检测芯片的研究  

Study on chip determining of F-of the metabolizability of fluoroacetamide

作　　者：常红发[1] 刘宪平[2] 张大明[2] 鞠熀先[3] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]北京市公安局法医检验鉴定中心,北京100192 [3]南京大学,江苏南京210093

出　　处：《中国法医学杂志》2010年第3期175-177,共3页Chinese Journal of Forensic Medicine

摘　　要：目的研制含氟毒物的检测芯片,实现氟乙酰胺等含氟毒物的快速电化学检测。方法利用氟离子选择电极制备含氟毒物的检测芯片,对芯片各项性能进行测试;建立氟乙酰胺染毒动物模型,使用制备的氟离子芯片检测染毒致死大白兔体内氟离子浓度。结果血液添加标准检测氟离子其线性响应范围为1～10-6mol/L,相关系数为0.9994,平均斜率接近理论斜率,检测限为5×10-7mol/L。染毒组大耳白兔血中氟离子含量较对照组高5～6倍(P<0.01),肝、肾、脑组织中氟离子含量较对照组高2～4倍(P<0.01)。结论此含氟毒物的检测芯片检测氟离子快速、简便、灵敏,可应用于氟乙酰胺等含氟毒物中毒案件的初筛。Objective To develop a determination chip with fluorine-containing poison in order to implement quickly electrochemical detection of the samples with fluoroacetamide and other fluorine-containing poison in the practical case. Methods The determination chip with fluorine-containing poison was prepared by using fluoride ion selective electrode and the performances of the chip were tested. The model of poisoned rabbit by fluoroacetamide was established,and then the concentration of fluoride ion in the sample was determined by the prepared chip with fluoride ion. Results The linear response range of the fluoride ion-selective chip for detection of fluoride ion was 1~10-6mol/L in adding a standard blood; the correlation coefficient,0.9994; an average slope,close to the theoretical slope; detection limit,5×10-7mol/L. The content of fluoride ion in blood of the poisoned rabbit was 5~6 times higher than the control group（P0.01）and 2~4 times in liver,kidney and brain（P0.01）. Conclusion It is fast,simple and sensitive to detect fluoride ion in the fluorine-containing poison by the chip,which can be applied for preliminary screening in the poisoning case with fluoroacetamide and other fluorine-containing poison.

关 键 词：法医毒物分析 氟乙酰胺 检测芯片 

分 类 号：D919[医药卫生—法医学]