桃PpMADS1基因启动子的克隆及功能分析  

Cloning and Function Analysis of the Promoter of PpMADS1 in Peach

作　　者：李翠[1] 谢华[1,2] 徐勇[2] 李云伏[1,2] 马荣才[1,2] 

机构地区：[1]首都师范大学生命科学学院,北京1000482 [2]北京农林科学院农业生物技术研究中心,北京100097

出　　处：《中国生物工程杂志》2010年第5期57-62,共6页China Biotechnology

摘　　要：PpMADS1基因属于一类MADS box基因,在植物的花发育调控中起着重要的作用。通过Genome Walking的方法从桃基因组中分离了长度为1814bp的PpMADS1基因启动子片段,序列分析表明,在此启动子上不仅含有TATA box和CAATbox基本元件,而且含有大量的与光调节有关的调控元件,如GT-1,Sp1和as-2-box,另外存在两个CArG-box元件、一个G-box元件和一个TGA-element,说明该启动子可能受光周期和激素的调控。将该启动子通过5′端缺失,分区段与GUS报告基因连接构建表达载体,并转化拟南芥。GUS组织化学染色分析结果表明,在-197到-454bp有促使GUS在花原基中表达的花原基特异性元件,在-454到-678bp之间存在促使GUS在萼片和花瓣表达的特异性元件,在-678到-978bp存在负调控作用元件,阻遏了GUS基因在花药中的表达。In order to analyze transcriptional regulative mechanism of PpMADS1 gene,the PpMADS1 promoter was obtained using Genome Walking method from peach（Prunus persica）genomic DNA.Sequence analysis indicated that there exist TATA-box,CAAT-box,two CArG box,one G-box,one TGA-element and a large number of regulatory elements involved in light response,such as GT-1,Sp1 and as-2-box,the results indicated that the promoter may be regulated by light and hormone.The PpMADS1 promoter was truncated according to the prediction of putative of cis-acting elements and fused with GUS reporter gene and transferred into Arabidopsis thaliana.Histochemical staining of different organs of the transgenic plants showed that the region between-197 to-454bp specified GUS expression in flower primordium and the region between-454 to-678bp in sepals and petals.A negatively regulatory element was shown to be present between-678 to-978bp that repressed GUS expression in filament.

关 键 词：桃 花发育 PpMADS1 启动子 顺式作用元件 

分 类 号：Q785[生物学—分子生物学]