基于模糊综合评价的产生物柴油微藻藻种筛选  

Application of Two-level Fuzzy Comprehensive Evaluation in Algae Screening for Biodiesel-producing Microalgae

作　　者：张齐[1] 郑洪立[1] 唐小红[1] 尹继龙[1] 高振[1] 纪晓俊[1] 李文琦[1] 黄和[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009

出　　处：《中国生物工程杂志》2010年第5期69-75,共7页China Biotechnology

基　　金：国家自然科学基金重点项目(20936002);国家"973"计划(2007CB707805;2009CB724700);江苏省六大人才高峰项目(2008);江苏省高校科研成果产业化推进项目(2009)资助项目

摘　　要：产生物柴油微藻大规模培养对微藻藻种的性能要求较高。从丰富的藻种资源中筛选到高品质的藻种一直是个亟待解决的问题。通过研究3株产油微藻,从系统工程的角度综合整个微藻生物柴油的技术工艺,建立了以生长速率、含油率、油脂组成等18种指标的二级评价体系,采用二级模糊综合评价的模糊数学方法对产生物柴油微藻的性能进行综和分析、筛选。最终确定供评价的三株微藻二级模糊综合评价集:小球藻LICME001[0.3600.3150.1920.0690.064],微绿球藻LICME002[0.2770.3310.2360.1040.052]和葡萄藻LICME003[0.3250.3710.2320.0710.060]。根据最大隶属度法则分析得:小球藻LICM001株产生物柴油微藻品质为优等级别,适合产生物柴油的技术工艺要求;微绿球藻LICME002和葡萄藻LICME003为良等级别的产生物柴油藻种。Large-scale culture has a high level requirement for the strain of microalgae,so screening of high quality microalgae strain has always been a problem to be settled.By researching three strains of biodiesel-producing microalgae,a two level estimate system which use 18 indicators such as the growth rate,oil content rate and oil composition has been built.Property of biodiesel-producing microalgae was analyzed using the method of two-level fuzzy comprehensive evaluation.And the result of the evaluation：Chlorella vulgaris LICME001[0.360 0.315 0.192 0.069 0.064],Nannochloropsis oculata LICME002[0.277 0.331 0.236 0.104 0.052] and Botryococcus Braunii LICME003[0.325 0.371 0.232 0.071 0.060].By the biggest membership principle,Chlorella vulgaris LICME001 were finally determented as high quality biodiesel-producing microalgae strain which is suitable for the requirement of biodiesel production technology.Nannochloropsis oculata LICME002 and Botryococcus Braunii LICME003 were determented as good quality biodiesel-producing microalgae.

关 键 词：微藻油脂 模糊综合评价 藻种筛选 生物柴油 

分 类 号：Q949.2[生物学—植物学]