耐碱的偶氮染料脱色菌筛选及其特性研究  

Screening and Characteristics of Azo Dye Decoloring Alkali-resistant Bacteria

作　　者：张胜琴[1] 陈必强[2] 杨光[3] 李春[4] 曹竹安[2] 

机构地区：[1]石河子大学化学化工学院,石河子832003 [2]清华大学化工系生物化工研究所,北京100084 [3]中国科学院天津工业技术生物研究所,天津300308 [4]北京理工大学生命学院,北京100081

出　　处：《中国生物工程杂志》2010年第5期76-80,共5页China Biotechnology

基　　金：国家"863"计划(2007AA021306)资助项目

摘　　要：从浙江某污水处理厂的活性污泥中筛选出若干株在高pH条件下对偶氮染料酸性大红GR有脱色能力的菌株,经脱色验证得到一株具有高效脱色活性的菌株Z1,经鉴定为巴斯德葡萄球菌（Staphylococcus pasteuri）,并对此菌株的脱色特性进行了初步研究。结果表明,在厌氧条件下,Z1在pH7-12,40h对50mg/L的酸性大红GR脱色率均可达90%以上。该菌株对染料有较强的耐受力,在酸性大红GR浓度为300mg/L时,48h的脱色率仍可达93%。此外,该菌株能够对多种偶氮染料脱色,具有较好的脱色广谱性,有望应用于处理工业废水中的偶氮染料。Studies were carried out to isolate Acid Scarlet GR resistant and decolored bacteria from sludge of a wastewater treatment plant,Zhejiang Province.Through dyeing decoloring tests,one strain,named Z1 was found to have a better decolorization performance at the high pH 12 and identified as Staphylococcus pasteuri by 16SrDNA gene sequence analysis.A strain named Z1 was screened through dye decolorization test and was identified as Staphylococcus pasteuri by 16SrDNA gene sequence analysis.And then the decolorization properties of Z1 were investigated.The results showed that the decolorization rate of 50mg/L Acid Scarlet GR could exceed 90% within 40h under anaerobic conditions at the range of pH 7~12,and the decolorization rate of 300mg/L Acid Scarlet GR even reached 93% within 48h.Moreover,the strain could effectively degrade other azo dyes with broad spectrum and could be applied for removing the azo dyes from the industrial wastewater.

关 键 词：偶氮染料 巴斯德葡萄球菌 耐碱菌 广谱性 

分 类 号：X788[环境科学与工程—环境工程]