微流体Coulter法测量细胞体积的数值分析  

Numerical Analysis of Cell Volumes Measured by Microfluidic Coulter Method

作　　者：何立群[1] 卢照升[1] 窦轩[1] 张海峰[1] 

机构地区：[1]中国科学技术大学热科学与能源工程系,合肥230027

出　　处：《中国生物医学工程学报》2010年第3期404-411,共8页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(50876100)

摘　　要：根据Coulter原理,电信号变化幅度对应着流过电极的细胞的体积。本研究数值分析了当细胞在微流体通道内流经布置在通道壁面上的电极时,电信号的变化规律;具体分析了电极形状和尺寸、细胞尺寸、细胞体积的渗透变化、细胞高度位置、细胞形状和微通道尺寸等因素对电极间电阻变化规律的影响。计算结果表明,体积恒定的细胞流经电极区域中心位置时,电阻变化幅度最大,而且这一变化幅度随细胞体积增大近似线性增大;而当细胞体积有渗透变化时,电阻变化曲线向体积较大的一端偏斜;细胞高度位置、细胞形状、微通道尺寸和电极尺寸对电阻变化最大幅度均有一定的影响。According to Coulter principle,the amplitudes of electric signal variation are corresponding to volumes of cells moving through the electrode zone.We numerical analyzed electric signal change rules when cells were passing through electrodes placed on the microchannel walls.Specifically,many factors that impact on impedance change rules were analyzed,including electrode geometries and dimension,cell size,osmosis variation of cell volume,cell height location,cell shape and microchannel dimension.The computing results showed that the amplitudes of impedance variation was maximum when the cell with constant volume was moving through the electrode zone center,and the amplitudes almost linearly increased with the increase of cell volume.But when the cell volume changed by osmosis,impedance variation curves skews to the end where the cell volume was larger.The cell height location,cell shape,microchannel size and electrode size all had some influence on the maximum amplitudes of impedance variation.

关 键 词：细胞体积 Coulter原理 微通道 

分 类 号：R318[医药卫生—生物医学工程]