小麦胚芽鞘长、幼苗根长的QTL定位  

QTL Mapping for Coleoptile Length and Root Length in Wheat Seedling

作　　者：李卓坤[1] 袁倩倩[1] 师翠兰[1] 陈俊男[1] 韩淑晓[1] 田纪春[1] 

机构地区：[1]山东农业大学国家作物生物学重点实验室小麦品质育种室,泰安271018

出　　处：《分子植物育种》2010年第3期460-468,共9页Molecular Plant Breeding

基　　金：国家重点基础研究计划(2009CB118301);国家自然科学基金(30971764);农业部行业科研基金(nyhyzx07-002)共同资助

摘　　要：小麦品种花培3号和豫麦57构建的DH群体的168个株系及亲本为材料,在正常发芽和20%PEG-6000模拟水分胁迫处理条件下测定小麦幼苗的胚芽鞘长、根长。利用完备区间作图法分析幼苗胚芽鞘长、幼根长的QTL。两种处理条件下共定位了8个控制胚芽鞘长加性QTL,其中位于染色体2A、4B和4D上的QCl2A、QCl4B和QCl4D在两种处理条件下均被检测到,可解释6.10%～16.31%的表型变异。两种条件下共定位了10个控制幼根长加性QTL,其中位于染色体6A上Xgwm82和Xwmc553区间的QRl6A在两种处理下均被检测到,可分别解释8.26%和9.74%的表型变异。在检测到的18对控制胚芽鞘长、根长的上位性互作位点中,大多数互作属于非等位QTL间的非加性QTL位点之间互作。因此在小麦材料的早期抗旱性筛选、分子育种时要同时考虑加性QTL和非加性QTL位点间的上位性互作。Taking Huapei 3,Yumai 57 and a DH（doubled haploid） population including 168 DH lines derived from Huapei 3 and Yumai 57 as materials,we have measured the coleoptile length,root length of wheat seedling under normal treatment and 20% PEG-6000 treatment.Inclusive composite interval mapping（ICIM） was applied to analyze the QTLs for coleoptile length and root length.As a result,we have detected eight additive QTLs for coleoptile length in total under the two treatments,Among them,QCl2A,QCl4B and QCl4D,located on chromosome 2A,4B and 4D,were detected both under the normal treatment and 20% PEG-6000 treatment,explaining 6.09%～16.31% of the phenotypic variation.We also have detected ten additive QTLs for root length,in which QRl6A,located in the interval between Xgwm82 and Xwmc553 on chromosome 6A,can be detected under the two treatments,explaining 8.26% and 9.74% of the phenotypic variation.Furthermore,we have detected eighteen pairs of epistatic QTLs for coleoptile length or for root length,and most of their epistatic interactions are between non-additive QTL and non-additive QTL.Therefore,the additive QTLs and epistatic interactions between no-additive QTLs should be both considered in early screening for drought resistance and marker-assisted breeding of wheat.

关 键 词：小麦 DH群体 胚芽鞘长 根长 加性QTL 上位性 

分 类 号：S512.1[农业科学—作物学]