一种同时评价小麦抗赤霉病侵入和扩展的策略  

A Strategy for Simultaneously Evaluating Resistance Against Initial Infection and Resistance Against Fungal Spread of Fusarium graminearum in Wheat

作　　者：薛树林[1] 安霞[1] 李国强[1] 许峰[1] 汪瑶[1] 唐明智[1] 贾海燕[1] 孔忠新[1] 马正强[1] 

机构地区：[1]南京农业大学应用植物基因组学与生物信息学研究中心,作物遗传与种质创新国家重点实验室,南京210095

出　　处：《分子植物育种》2010年第3期478-482,共5页Molecular Plant Breeding

基　　金：国家973项目(2010CB125902);国家自然科学基金项目(30430440;30025030;30671295;30721140555);江苏省自然科学基金项目(BK2009305)共同资助

摘　　要：抗侵入(typeⅠ)和抗扩展(typeⅡ)是小麦对赤霉病抗性的两种主要类型,其抗性机制迥异,接种方法和抗性鉴定指标也完全不同。为了建立一种同时评价这两种抗性的策略,本研究以喷雾接种后14d和21d的病小穗数(NDS14和NDS21)以及这两种数据的差值(DNDS)作为抗性鉴定指标。利用从南大2419×望水白重组自交系群体中所获得表型数据进行QTL定位,结果表明,NDS14主要反映抗侵入性,DNDS主要反映抗扩展性,这说明在同一试验中同时评价抗赤霉病侵入和扩展这两种抗性是可行的。与分别进行这两种抗性鉴定的方法相比,本研究所提出的策略所需资源更少、更省时省力,可广泛用于小麦抗赤霉病育种以及抗性遗传分析的研究。Resistance to Fusarium head blight（FHB） or scab in wheat mainly exhibit itself in two types：Resistance against initial infection（typeⅠ） and resistance against fungal spread（typeⅡ）,which are different in inoculation methods,resistance mechanisms,and resistance evaluation index.To establish a strategy suitable for evaluating the two types of resistances simultaneously,the number of diseased spikelets collected on the 14th and 21st day post spraying inoculation（NDS14 and NDS21）,and the difference between them（DNDS） were used as evaluation index to measure scab resistance.QTL mapping results based on the phenotypic data from the recombinant inbred line（RIL） population derived from Nanda2419×Wangshuibai indicated that NDS14mainly reflected typeⅠ resistance,and that DNDS mainly reflected typeⅡ resistance,suggesting it is feasible to simultaneously evaluate typeⅠ resistance and typeⅡ resistance in a single trial.Compared with the approaches separately evaluating the two resistance types,this new strategy turned to be resource-saving,time-saving and effort-saving,thus could be widely used in breeding as well as genetic analysis of scab resistance of wheat.

关 键 词：赤霉病 抗侵入 抗扩展 抗性鉴定指标 

分 类 号：S512.1[农业科学—作物学]