高等植物蛋白质的特异性降解系统  

Protein Specific Degradation Systems in Higher Plants

作　　者：杜国华[1] 张立军[1] 樊金娟[1] 阮燕晔[1] 刘淳[1] 许红梅[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110866

出　　处：《分子植物育种》2010年第3期567-576,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(30870190);教育部博士点基金项目(200801570005);辽宁省科技项目(2008208001);辽宁省高校科研项目(2008S211);沈阳市科技项目(1081191-3-00)共同资助

摘　　要：在细胞内功能蛋白质处于不断的合成与降解中,其中蛋白质的特异性降解是蛋白功能调节的重要机制。蛋白质的特异性降解分为泛素依赖的途径和非泛素依赖途径。在泛素依赖的降解途径中,蛋白质在泛素激活酶、泛素结合酶、泛素连接酶的作用下泛素化,然后在26S蛋白酶体中进行降解。蛋白质也可不依赖泛素被26S蛋白酶体降解,或被游离的20S蛋白酶体降解。本文主要介绍高等植物中的蛋白质特异性降解途径及特异性机制的研究进展。Functional proteins are continually being synthesized and degraded in cells.The protein specific degradation is an important regulation mechanism of protein function.The protein specific degradation systems include the ubiquitin-dependent proteolysis and ubiquitin-independent proteolysis.In the ubiquitin-dependent proteolysis,the substrate proteins are ubiquitylated under the action of the ubiquitin-activating enzyme,the ubiquitinconjugating enzyme and the ubiquitin ligase,and then degraded in 26S proteasome.The proteins can also be degraded by the 26S proteasome without prior ubiquitination or by the free 20S proteasome directly.This paper reviews the progress of the pathway and mechanism of protein specific degradation in plants.

关 键 词：泛素化 泛素连接酶 去泛素化酶 蛋白酶体 N末端规则 

分 类 号：Q946.1[生物学—植物学]