太子参组织培养与快繁技术  

Study on the tissue rapid propagation technique of pseudostellaria heterophylla Miq. Pax

作　　者：宋丽莎[1] 黎娇凌[1] 肖国学[1] 刘丽萍[1] 罗应[1] 钱绍方[1] 

机构地区：[1]黔南民族师范学院生命科学系,贵州都匀558000

出　　处：《广东农业科学》2010年第6期55-57,共3页Guangdong Agricultural Sciences

基　　金：黔南州中药现代化专项重点实验室项目(2009)

摘　　要：以太子参冬芽为外植体进行组织培养试验,结果表明,1/2MS+NAA0.1mg/L+6-BA2.0～3.0mg/L能诱导太子参越冬芽的顶芽和腋芽生长;1/2MS+NAA0.2mg/L+6-BA1.0mg/L适宜诱导丛生芽生长;1/2MS+NAA0.2mg/L+6-BA1.5mg/L适宜太子参组培苗增殖继代培养;MS基本培养基适宜太子参壮苗生根。The winter buds excised from pseudostellaria heterophylla Miq. pax was used as explants for tissue culture in this test. The results showed that 1/2MS＋NAA0.1 mg/L＋6-BA2.0～3.0 mg/L medium could induce the growth of overwintering terminal buds and axillary buds. 1/2MS＋NAA0.2 mg/L＋6-BA1.0 mg/L was the best medium for the induction of multiple shoot. 1/2MS＋NAA0.2 mg/L＋6-BA1.5 mg/L was suitable for the proliferation of tissue culture plantlets. And MS culture medium was suited for sound seedling to take roots.

关 键 词：太子参 冬芽 组织培养 快繁技术 

分 类 号：S567.53[农业科学—中草药栽培]