生物信息学技术在结核分支杆菌ESAT-6重组抗原研究中的应用  

Application of Bioinformatics in the Study of Mycobacterium Tuberculosis Recombinant Antigen ESAT-6

作　　者：丁淑琴[1] 史彦奎[1] 杨圆圆[2] 杨凤琴[1] 

机构地区：[1]宁夏医科大学,银川750004 [2]银川市第一人民医院,银川750001

出　　处：《宁夏医科大学学报》2010年第3期334-336,F0003,共4页Journal of Ningxia Medical University

基　　金：宁夏医科大学校级项目基金资助(项目编号:XM200825)

摘　　要：目的应用生物信息学分析软件预测分析结核分支杆菌ESAT-6基因及相关蛋白的特性。方法应用NCBI、Expasy等在线生物信息学网站及DNAstar、Rasmol等软件包分析ESAT-6并进行同源比对;预测二级、三级结构,以及预测主要抗原表位等。结果结核分支杆菌重组抗原ESAT-6与已发表氨基酸序列同源性为90%。预测该蛋白分子质量约为9885.7Da,PI为4.6,4个抗原表位,其结构域位于56-87位。结论生物信息学技术在结核分支杆菌ESAT-6重组抗原研究中有一定的理论和应用价值。Objective To predict the structure and function of recombinant antigen ESAT-6 of Mycobacterium tuberculosis using bioinformatics method.MethodsBy online analysis at bioinformatics websites such as NCBI（http：//www.nibi.nlm.nih.gov/）and Expasy（http：//cn.expasy.org/）,and employing software packages such as DNAstar and Rasmol to do multi-sequence homological alignment,secondary structure and tertiary structure,antigenic epitope analysis,etc.ResultsCompared with the amino acid sequence of ESAT-6 of Mycobacterium tuberculosis published,the homologies was 90%.Analysis of the predicted protein indicated a molecular mass of 9885.7 KDa,PI was 4.6,function sites and four antigen pitope were found.Conclusion Bioinformatic is valuable to the study ESAT-6 of Mycobacterium tuberculosis.

关 键 词：生物信息学 结核分支杆菌 ESAT-6 重组抗原 

分 类 号：R392[医药卫生—免疫学]