含β射线的软性血管外模型对移植血管内膜增生及平滑肌增殖的抑制作用  

Soft extravascular model (SEM) with β radioactive ray inhibits smooth muscle cell proliferation and intimal hyperplasia in autogenous vein graft

作　　者：钱济先[1,2,3,4] 钱兆奇[1,2,3,4] 黄耀添[1,2,3,4] 李桂云 王剑波[1,2,3,4] 胡佩珍 王军[1,2,3,4] 吕荣 

机构地区：[1]第四军医大学西京医院全军骨科研究所 [2]上海第二医科大学附属仁济医院血管外科 [3]西京医院妇产科 [4]安徽省宿县地区人民医院骨科

出　　处：《中华医学杂志》1999年第1期19-21,共3页National Medical Journal of China

摘　　要：目的观察含β射线３２磷（３２Ｐ）的软性血管外模型（ＳＥＭ）对移植血管内膜增生（ＩＨ）及平滑肌细胞（ＳＭＣ）增殖的抑制作用。方法作者在２４只家兔股静脉———股动脉移植模型上，应用内皮素Ⅰ受体基因（ＥＴＡｃＤＮＡ）原位分子杂交及计算机图像分析技术，在不同条件下（分对照组、阿斯匹林（ＡＳＰ）组和３２Ｐ组），术后（１、２、４、１２周）不同时间内定量观察ＥＴＡｃＤＮＡ表达情况及内膜相对厚度。结果（１）含β射线的ＳＥＭ具有显著抑制移植血管ＳＭＣ增殖的作用，其作用可延续至移植术后１２周。（２）含β射线的ＳＥＭ对于早、中期（１～４周）移植血管内膜增生具有显著的抑制作用。（３）ＡＳＰ在本实验条件下并无明显的抑制ＳＭＣ增殖及ＩＨ的作用。结论含β射线３２Ｐ的ＳＥＭ对移植血管内膜增生及ＳＭＣ增殖有显著抑制作用。Objective To investigate the effects of the soft extravascular model (SEM) with 32 P on intimal hyperplasia and smooth muscle cell preliferation in autogenous vein grafts. Methods We transplanted the femoral veins to the co lateral femoral arteries in rabbits. The animals were divided into three groups of Aspirin group, 32 P group and control group. We observed the expression density of endothelin type A receptor cDNA (ETAcDNA) and intimal relative thickness by using in situ molecular hybridization of ETAcDNA by computerized image analysis technology. Results SEM with β ray obviously inhibited SMC preliferation from 1 W to 12 W postoperation, and notably restrained intimal hyperplasia at early to middle stage of graft. Aspirin could not inhibit SMC proliferation and intimal hyperplasia under the condition of this experiment.

关 键 词：移植血管 血管内膜增生 平滑肌增殖 磷32 

分 类 号：R654.3[医药卫生—外科学]