流感嗜血杆菌的蛋白电泳图谱分析及分型  

Analysis of the protein of Haemophilus influenzae

作　　者：解静平[1] 薛冰[1] 王丽红[1] 袁艺[1] 曹玉璞[1] 

机构地区：[1]首都儿科研究所细菌研究室

出　　处：《中华医学杂志》1999年第1期61-63,共3页National Medical Journal of China

摘　　要：目的分析流感嗜血杆菌蛋白的成分及免疫原性。为流感嗜血杆菌菌苗的研制提供依据。方法应用聚丙烯酰胺凝胶电泳分析５６株流感嗜血杆菌蛋白成分。结果５６株流感嗜血杆菌中不可分型流感嗜血杆菌４４株，占７９％。生物分型中，以Ⅱ型为最高，占４８％。流感嗜血杆菌约有２０多条蛋白带，１１条为所有型别菌株共同具有的，其中以相对分子量为９６０００、５１０００及３１０００者含量较高。各菌株间的差异蛋白的相对分子量主要为３１０００～５１０００，但不同生物型别间的蛋白差异无显著相关。结论流感嗜血杆菌具有共同的蛋白成分，这为制备其所有型别共同菌苗提供了依据。Objective To understand Haemophilus influenzae special protein and to help for preparing Haemophilus influenzae Vaccine. Methods Proteins from isolates of Haemophilus influenzae were examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Results 23 isolates were from nasopharyngeal secretions of children with pneumonia and 33 from pharyx swab of healthy children. Conventional tests showed that 79% of fifty six strains of Haemophilus influenzae were non serotypable and all belonged to one of eight biotypes. The common biotypes were Ⅱ (48%). All strains contained more than 10 proteins with molecular weights of 96 000, 86 000, 55 000, 51 000, 46 000, 40 000, 39 000, 31 000, 29 000, 25 000, and 16 000. Among them, three were major proteins (31 000, 51 000 and 96 000) and different proteins were mainly between peptide 31 000 and 51 000. A possible association between biotype and protein was unclear. Conclusion Analysis of protein of Haemophilus influenzae is helpful for epidemiological study of non serotypable Haemophilus influenzae and making Haemophilus influenzae vaccine.

关 键 词：流感嗜血杆菌 蛋白电泳 图谱分析 分型 

分 类 号：R378.41[医药卫生—病原生物学]