染尘大鼠肺泡灌洗液对人胚肺细胞DNA含量的影响  

Fffect of Bronchoalveolar Lavage Fluid (BALF)from Rats Expousure to Chrysotile or Silica on DNA Content of Human Embryo Lung Cell(HEL)

作　　者：梅瑜[1] 吴卫东[1] 刘世杰[1] 王起恩[1] 樊晶光[1] 

机构地区：[1]北京医科大学劳动卫生学教研室

出　　处：《中国公共卫生》1999年第1期22-23,共2页Chinese Journal of Public Health

摘　　要：用流式细胞仪测定了石棉和石英染尘后２个月内大鼠支气管肺泡灌洗液对人胚肺细胞ＤＮＡ含量水平的影响。结果发现石棉和石英均可引起灌洗液中促进ＤＮＡ合成物质的含量增加，石棉诱导这种促ＤＮＡ合成的能力比石英更强、更持久，提示石棉诱导这种生长因子高度的表达，可能在石棉的致肿瘤过程中起一定作用。groups of male Wistar rats were intratracheally instilled with 1ml sailine,Mang Ya chrysotile suspension(10mg/ml),or silica suspension(10mg/ml)per rat,respectively.Rats were sacrificed 10,30 and 60days after exposure and the effect was characterized with flow cytometer by analysis of bronchoalvcolar lavagc fluid (BALF)on DNA contcnt of Human Embryo Lung cell(HEL).The results showed that the BALF from rats explosure to chrysotile or silica increase DNA content of HE,but the increase in chrysotile group was stronger and persisted longer than in silica group,it may be related with carcinotenesis of chrysotile.

关 键 词：石棉 石英 肺灌洗 肺细胞 DNA 尘肺 

分 类 号：R135.2[医药卫生—劳动卫生]