胸腺肽α1体外刺激胃癌患者外周血单个核细胞对淋巴细胞亚群影响  

Effect of thymosin α1 on percentage of T-lymphocyte subsets in in vitro peripheral blood mononuclear cells from gastric cancer patients

作　　者：杨霞[1] 钱锋孙[2] 刘凯军[1] 何海洋[1] 兰远志[2] 田易[1] 傅晓岚[1] 李健[1] 张记[1] 申子刚[1] 李晋涛[1] 吴玉章[1] 

机构地区：[1]第三军医大学基础医学部全军免疫研究所,重庆400038 [2]第三军医大学西南医院普通外科,微创胃肠外科中心,重庆400038

出　　处：《第三军医大学学报》2010年第12期1249-1252,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30972716)~~

摘　　要：目的观察胸腺肽α1(thymosin α1,Tα1)体外刺激培养胃癌患者外周血单个核细胞(peripheral blood mono-nuclear cells,PBMCs)对淋巴细胞亚群分化及分泌细胞因子谱的影响,分析Tα1对肿瘤患者免疫功能的影响。方法分离32例胃癌患者及18例健康自愿者PBMCs,50、10、1μg/mlTα1体外刺激培养2d;流式细胞仪检测CD4+、CD8+、CD4+CD25+Foxp3+T细胞百分比、CD4+/CD8+变化及分泌Th1/Th2细胞因子谱变化。结果 Tα1体外刺激PBMCs后,胃癌患者及健康自愿者的CD4+、CD8+T细胞水平及CD4+/CD8+比值均无明显变化;健康自愿者CD4+CD25+Foxp3+T细胞水平无明显变化,胃癌患者CD4+CD25+Foxp3+T细胞水平明显提高(P<0.05);1μg/mlTα1促进胃癌患者PBMCs分泌IL-1β、TNF-α,10μg/ml促进胃癌患者PBMCs分泌IL-6(P<0.05),健康自愿者PBMCs分泌细胞因子谱变化不明显(P>0.05)。结论 Tα1体外刺激胃癌患者PBMCs,提高CD4+CD25+Foxp3+Treg细胞亚群水平,可能对肿瘤患者抗肿瘤免疫起抑制效应。Objective To investigate the effect of thymosin α1 （Tα1） on cellular immune function in gastric cancer patients through observing its treatment on the differentiation of T-lymphocyte subsets from screened peripheral blood mononuclear cells （PBMCs）. Methods PBMCs were obtained by centrifugation of blood samples from 18 healthy subjects and 32 patients with gastric cancer,and then cultured in the presence of culture medium with addition of Tα1 at 50,10 and 1 μg/ml for 2 d. T lymphocyte subsets （such as CD4＋,CD8＋ and CD4＋ CD25＋ Foxp3＋ T cells） and Th1/Th2 multiplex cytokines were detected by flow cytometry （FCM）. Results After PBMCs isolated from healthy people and patients were incubated with or without Tα1,there was no significant change in percentage of CD4＋,CD8＋ peripheral lymphocyte subsets and ratio of CD4＋/CD8＋. There was no obvious change in the percentage of CD4＋ CD25＋ Foxp3＋ T lymphocyte subsets in the normal control,but a significant increase was observed in the cells from patients with gastric cancer after treatment （P0.05）. Tα1 at 1 μg/ml induced the secretions of IL-1β and TNF-α in PBMCs from patients with respect to PBS treated cells,and 10 μg/ml of Tα1 increased the secretion of IL-6 in patient PBMCs. However,Tα1 had no such effect on multiplex cytokines in PBMCs from healthy people. Conclusion Tα1 increases the percentage of CD4＋ CD25＋ Foxp3＋ Treg cells in PBMCs from gastric cancer patients,and it may increase risk of patients for tumor immunotolerance.

关 键 词：胸腺素 单核细胞 胃肿瘤 淋巴细胞亚群 TH1细胞 TH2细胞 

分 类 号：R392.12[医药卫生—免疫学] R735.2[医药卫生—基础医学]