脂质代谢相关基因在乳腺浸润性导管癌中的表达及其意义  

Expression and significance of lipid metabolism-related genes in breast invasive ductal carcinoma

作　　者：吕静[1] 王斌[1] 糜漫天[1] 张乾勇[1] 朱俊东[1] 王建[2] 

机构地区：[1]第三军医大学军事预防医学院营养与食品卫生学教研室,营养与食品安全研究中心,重庆400038 [2]第三军医大学新桥医院营养科,重庆400037

出　　处：《第三军医大学学报》2010年第12期1290-1292,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30901195);达能膳食营养研究与宣教基金(DIC2009-08)~~

摘　　要：目的观察乳腺浸润性导管癌组织和癌旁正常乳腺组织中脂质代谢调控基因的表达,并探讨其与乳腺浸润性导管癌发生的关系。方法收集30例乳腺浸润性导管癌患者手术切除的乳腺癌组织和癌旁乳腺组织,RT-PCR检测各组织中脂肪酸合成酶(fatty acid synthase,FAS)、环氧合酶(cyclooxygenase-2,Cox-2)、5-脂氧合酶(5-lipoxygenase,5-LOX)及过氧化物酶增殖活化受体γ(peroxisome proliferator-activated receptorγ,PPAR-γ)的基因表达。结果乳腺浸润性导管癌组织中FASmRNA的表达显著高于癌旁组织(3.265±0.913vs1.300±0.712,P<0.01),同时乳腺癌组织中Cox-2、5-LOXmRNA的表达显著高于癌旁组织(2.449±1.333vs1.027±0.596,P<0.05;2.766±1.193vs1.159±0.520,P<0.05),PPAR-γmRNA的表达在乳腺癌组织和癌旁乳腺组织间差异无统计学意义(2.771±1.313vs2.235±1.383,P>0.05)。结论 FAS、Cox-2及5-LOX基因的高表达与乳腺浸润性导管癌的发生有关,提示脂质代谢途径调控对乳腺癌的预防具有重要的意义。Objective To investigate the expressions of lipid metabolism-related genes in breast invasive ductal carcinoma and adjacent non-cancerous tissues,and evaluate their roles in the occurrence of breast invasive ductal carcinoma. Methods Experimental samples were derived from our previous population study on breast cancer. Breast cancer tissues and adjacent non-cancerous tissues were spontaneously collected from 30 invasive ductal carcinoma patients. The expressions of fatty acid synthase （FAS）,cyclooxygenase-2 （Cox-2）,5-lipoxygenase （5-LOX） and peroxisome proliferator-activated receptor γ （PPAR-γ） mRNA were detected by RT-PCR. Results Expressions of FAS,Cox-2,and 5-LOX in breast cancer tissues were significantly higher than those in adjacent tissues （FAS：3.265±0.913 vs 1.300±0.712,P0.01; Cox-2 and 5-LOX：2.449±1.333 vs 1.027±0.596,P0.05; 2.766±1.193 vs 1.159±0.520,P0.05）. There was no significant difference of PPAR-γ expression between the 2 groups （2.771±1.313 vs 2.235±1.383,P0.05）. Conclusion Higher expressions of FAS,Cox-2,and 5-LOX are positively correlated with the occurrence of breast invasive ductal carcinoma.

关 键 词：癌 导管 乳腺 脂肪酸合成酶复合物 环氧化酶2 花生四烯酸盐5-脂氧合酶 过氧化物酶增殖活化受体γ 

分 类 号：Q591.5[生物学—生物化学] R349.3[医药卫生—基础医学]