高压冷冻技术在拟南芥细胞微结构研究中的应用  

Application of high pressure freezing technique in structural study of Arabidopsis thaliana

作　　者：王广超[1] 冯振华[1] 孙旭东[1] 郭建胜[1] 祝建[1] 

机构地区：[1]同济大学生命科学与技术学院,上海200092

出　　处：《电子显微学报》2010年第2期152-162,共11页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.30970169)

摘　　要：高压冷冻技术是指以高压冷冻固定为主要内容的一种电镜技术。该技术可以充分、快速固定样品的同时,避免引入操作干扰,使得电镜结构更接近样品生活状态。本文利用拟南芥为材料,比较了高压冷冻固定及冷冻替代制备的电镜样品同常规化学固定、室温梯度脱水方法所制备样品的差异。结果显示,在拟南芥根及叶片中,高压冷冻技术可以有效地避免常规制备样品中的细胞壁收缩,细胞质不均匀分布,高尔基体及质体收缩,细胞膜及液泡膜皱缩甚至断裂等现象,使细胞结构更接近其生活时的状态。The high pressure freezing technology can fully and quickly fix samples avoiding the introduction of operational artifact and making the structure close to living conditions.In this paper,the samples of Arabidopsis thaliana prepared by high-pressure freezing and freeze substitution fixation are compared with conventional chemical method.The results showed that the samples （ roots and leaves of Arabidopsis ） prepared by high pressure freezing can effectively avoid the defects in routine sample preparation such as cell wall shrinkage,uneven distribution of cytoplasm and the shrinkage of Golgi body,Golgi vesicles,cell membrane and vacuole membrane or even rupture phenomena,and make the cell structures close to their living state.

关 键 词：高压冷冻 冷冻替代 拟南芥 微结构 脱分化 

分 类 号：Q942[生物学—植物学] Q336