土牛膝中齐墩果酸、牛膝多糖的提取与鉴定  

Extracting and Identification of OA and ABPS of local achyranthes aspera

作　　者：黄志芳[1,2] 黄志红[1] 胡吉林[1] 周跃辉[1] 魏得良[1] 

机构地区：[1]湘南学院化学与生命科学系,423000 [2]广州市第十二人民医院

出　　处：《中国实用医药》2010年第17期6-7,共2页China Practical Medicine

基　　金：湖南省教育厅科研基金资助项目(项编号目:05C630);湖南省科技厅科研基金资助项目(项编号目:06FJ4099)

摘　　要：目的从土牛膝中提取齐墩果酸和多糖并进行定性定量分析。方法土牛膝水煮液经水浴、酸水解、加碱煮沸后,乙醇回流、抽滤,60℃烘干精制得到齐墩果酸;土牛膝的乙醇热回流后再水提,经过浓缩、沉淀、除杂、抽滤、洗涤、低温干燥得到牛膝多糖。用薄层色谱法和分光光度法进行定性定量分析。结果齐墩果酸的提取率为0.88%;牛膝多糖的提取率为7.2%。结论通过含量测定结果与提取率对比,本实验方法可行。Objective To extract oleanolic acid （OA） and achyranthes bidentata polysaccharides （ AB- PS） from local achyranthes aspera, and analyze quantitatively and qualitatively. Methods The oleanolic acid （OA） was obtained from the decoction of local achyranthes aspera through the process of washing, acid hydroly- sising, adding alkali and boiling; ethanol refluxing, vacuum filtrating and 60℃ drying. After the ethanol hot re- fluxing and aqueous extracting, the achyranthes bidentata polysaccharides （ABPS） are obtained through the process of condensing, sedimenting, impurities removing, vacuum filtrating, washing, cooling and drying. Ul- traviolet spectroscopy and TCL are adopted for qualitative and quantitive analysis. Results The extracting rate of OA and ABPS were 0. 88% and 7.2% respectively. Conclusion The experimental method of comparising the content determination and the extracting rate is easy and feasible.

关 键 词：土牛膝 齐墩果酸 牛膝多糖 提取与鉴定 

分 类 号：R284.1[医药卫生—中药学]