抗核小体抗体对系统性红斑狼疮的诊断意义  

Diagnostic significance of anti-nucleosome antibodies in systemic lupus erythematosus

作　　者：许方[1] 初礼巍[2] 

机构地区：[1]大连医科大学附属第二医院检验科,辽宁大连116027 [2]大连医科大学检验医学院,辽宁大连116044

出　　处：《大连医科大学学报》2010年第3期335-338,共4页Journal of Dalian Medical University

摘　　要：[目的]探讨抗核小体抗体(AnuA)的检测对系统性红斑狼疮(SLE)的诊断价值及其与SLE活动性的关系。[方法]选择SLE患者60例、疾病对照组30例和正常对照组30例,采用ELISA方法测定血清AnuA,同时检测并分析抗ds-DNA抗体、抗Sm抗体两项实验室指标。[结果]AnuA、抗ds-DNA抗体和抗Sm抗体在SLE患者中阳性率分别为75.00%、50.00%、35.00%,均明显高于疾病对照组(10.00%、6.67%、3.33%)及正常对照组(0、0、0),P均<0.05。AnuA对SLE诊断的敏感性为75.00%,与抗ds-DNA抗体(50.00%)和抗Sm抗体(35.00%)比较差异有显著性意义(P<0.05),特异性为90.00%,与抗ds-DNA抗体和抗Sm抗体比较差异无显著性意义(P>0.05)。活动期AnuA阳性率明显高于非活动期,AnuA在活动期的阳性率(91.7%)高于抗ds-DNA的阳性率(66.7%),AnuA、抗ds-DNA抗体和抗Sm抗体联合测定的敏感性为91.67%。[结论]AnuA的检测在SLE患者中表现为出现时间早,特异性强,敏感性高,可以作为临床早期诊断SLE的重要指标之一,且与SLE疾病活动性有关。与抗ds-DNA抗体、抗Sm抗体联合检测有助于提高诊断敏感性。[Objective] To explore the diagnostic value of anti-nucleosome antibodies（AnuA） and the relationship with activity of systemic lupus erythematosus（SLE）.[Methods] AnuA in the serum of 60 SLE patients,30 disease control group and 30 normal control group were tested by enzyme linked immunosorbent assay（ELISA） method,at the same time anti-ds-DNA antibody and anti-Sm antibody were detected and analyzed.[Results] The positive rate of AnuA,anti-ds-DNA antibody and anti-Sm antibody in SLE were 75.00%,50.00% and 35.00%.They were higher than disease control（P0.05） and normal control（P0.05） groups.The sensitivity of AnuA was 75.00% in SLE and it was difference with anti-ds-DNA antibody and anti-Sm antibody（P0.05）.The specificity of AnuA was 90.00%,but it had no difference with them（P0.05）.In addition AnuA in active stage was significantly higher than that in non-active stage.The positive rate of AnuA in active stage was higher than anti-ds-DNA antibody.The sensitivity of combination of AnuA and anti-ds-DNA antibody and anti-Sm antibody was 91.67%.[Conclusion] AnuA in SLE can contribute to earlier diagnosis.It has high sensitivity and specificity and can act as marker of clinical diagnosis of early stage in SLE.Combination of AnuA,anti-ds-DNA antibody and anti-Sm antibody can increase the clinical diagnosis sensitivity.

关 键 词：抗核小体抗体 红斑狼疮 系统性 

分 类 号：R593.241[医药卫生—内科学]