孕期酒精暴露对子鼠视皮质突触数量影响的体视学研究  

Stereological study on the synapse loss in visual cortex of mouse after prenatal alcohol exposure

作　　者：席艳[1] 张俊士[1] 臧建峰[1] 文曙光[1] 邓锦波[1] 

机构地区：[1]河南大学神经生物学研究所,河南开封475004

出　　处：《药学学报》2010年第6期705-710,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30771140);河南省教育厅自然科学研究项目(2007180008);河南省科技厅国际协作项目(094300510044);河南大学自然科学基础研究项目(2008YBZR034)

摘　　要：建立孕期酒精暴露(PAE)模型,研究孕期酒精暴露对小鼠视皮质突触数量的影响。利用免疫荧光染色技术标记对照组与PAE模型组(低剂量和高剂量)子鼠出生后0、7、14及30d视皮质突触前体的synaptophysin蛋白表达,以此来代表突触,观察其数密度变化,并利用Western blotting检测对各实验组子代小鼠视皮质synaptophysin的表达量进行半定量分析。突触数密度值统计学分析显示:对照组、低剂量和高剂量组间比较差异显著(P<0.05),0、7、14及30d差异显著(P<0.05),年龄与剂量之间存在交互作用(P<0.05)且剂量的影响作用更大。Western blotting检测结果与免疫荧光统计结果一致。这表明PAE对突触的影响具有长时程效应和剂量相关性,突触丢失的长时程放大效应可能是患儿精神发育迟滞和记忆力下降的主要原因。In order to understand the alcohol＇s toxicity to the quantitative alternations of synapses in mouse visual cortex,the expression of synaptophysin after prenatal alcohol exposure was investigated.In present study,the experimental mice at P0,P7,P14 and P30 were grouped,as control,2 g·kg·1 alcohol treatment and 4 g·kg·1 alcohol treatment.The pre-synaptic elements which were used to represent synapses were marked with synaptophysin（a synaptic vesicle associated protein） by immunocytochemistry technique.The synaptophysin positive boutons in layer VI of visual cortex were imaged under laser confocal microscope.With stereological methods,the number cal density of synapse in visual cortex was calculated in different groups at various ages.Moreover,Western blotting was carried out to detect the expression of synaptophysin in visual cortex.The results showed that prenatal alcohol exposure could cause synaptic loss with long-term effect and in a dose dependent manner.For instance,there were significant difference among the different treatment groups of P0,P14 and P30 as well（P 0.05）.Western blotting supported the results of immunofluorescent labeling.In conclusion,prenatal alcohol exposure can induce the synaptic loss dose dependently and with long-term effect.Our findings implicate that the synaptic loss with long term effect in CNS probably contributes to the lifelong mental retardation and memorial lowliness associated with childhood FAS.

关 键 词：孕期酒精暴露 数密度 突触 SYNAPTOPHYSIN 视皮质 

分 类 号：R963[医药卫生—微生物与生化药学]