贯众总多糖对空肠弯曲杆菌诱导的系统性红斑狼疮样综合征小鼠的作用  

Effect of Matteuccia struthiopteris polysaccharides on systemic lupus erythematosus-like syndrome induced by Campylobacter jejuni in BALB/c mice

作　　者：王铮[1] 谢俊云[1] 徐晗[2] 程小芹[1] 乐曦玲[1] 力弘[1] 章蕴毅[1] 卢燕[2] 陈道峰[2] 

机构地区：[1]复旦大学药学院药理学教研室,上海201203 [2]复旦大学药学院生药学教研室,上海201203

出　　处：《药学学报》2010年第6期711-717,共7页Acta Pharmaceutica Sinica

基　　金：上海市科委中药现代化专项项目(08DZ1971202);国家杰出青年科学基金项目(30925042);科技部"重大新药创制"科技专项课题(2009ZX09502-013)

摘　　要：观察贯众总多糖(Ms)对空肠弯曲杆菌CJ-S131诱导的红斑狼疮样综合征小鼠的防治作用。BALB/c小鼠随机分为6组,即正常组、模型对照组、SLE模型组、贯众总多糖(30和15mg·kg·1)组以及泼尼松(5mg·kg·1)组,采用CJ-S131免疫诱导小鼠疾病模型。检测贯众总多糖对小鼠体重和脏器指数、对抗自身抗体和总IgG水平以及尿蛋白和肾损伤的影响。结果表明,与SLE模型组相比,贯众总多糖可显著改善SLE模型小鼠体重降低(P<0.05),显著降低小鼠抗自身抗体和总IgG水平、抑制尿蛋白的升高,改善肾病理损伤。结果表明,贯众总多糖对狼疮样综合征小鼠有保护作用。Matteuccia struthiopteris is a nature plant,which contains a lot of potential active components.In the present study,we investigated the effect of polysaccharides extracted from Matteuccia struthiopteris on lupus-like syndrome induced by Campylobacter jejuni CJ-S131 in BALB/c mice.Mice were randomly divided into normal,model control,SLE model（vehicle treated）,Matteuccia struthiopteris polysaccharides treated（30 and 15 mg·kg-1） groups and prednisone 5 mg·kg-1 treated groups.The effect of Matteuccia struthiopteris polysaccharides（Ms） on weight and organ index of BALB/c mice was detected.Autoantibodies and total IgG production were measured by enzyme linked immunosorbent assay.Proteinuria was measured and kidneys were examined by light microscopy.Compared with SLE model group,treatment with Matteuccia struthiopteris polysaccharides 30 and 15 mg·kg-1 reduced weight loss and Matteuccia struthiopteris polysaccharides 15 mg·kg-1 reduced spleen swelling（P 0.05）.The increased production of autoantibodies and total immunoglobulin G（IgG） were also significantly inhibited.Matteuccia struthiopteris polysaccharides protected kidney against glomerular injury in BALB/c mice with reduced immunoglobulin deposition and lowered proteinuria（P 0.01）.Matteuccia struthiopteris polysaccharides had a protective effect on lupus-like syndrome induced by CJ-S131 in BALB/c mice.

关 键 词：贯众总多糖 抗自身抗体 尿蛋白 肾损伤 系统性红斑狼疮 

分 类 号：R285[医药卫生—中药学]