The effect of the rice blast resistance gene Pi36 on the expression of disease resistance-related genes  

作　　者：LIU XinQiong LI YuanYuan WANG LiYuan LIU XueQun WANG ChunTai WANG Ling PAN QingHua 

机构地区：[1]Key Biotechnology Laboratory of State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan 430074, China [2]Laboratory of Plant Resistance and Genetics, College of Resources and Environmental Sciences, South China Agricultural University, Guangzhou 510642, China

出　　处：《Chinese Science Bulletin》2010年第18期1881-1888,共8页

基　　金：supported by the National Natural Science Foundation of China (30600400);the Chen-Guang Program of the Wuhan City (200750731302);the National Industry Research Project (200803008)

摘　　要：The resistance gene Pi36 confers a stable and high level of resistance against Chinese isolates of the rice blast pathogen Mag-naporthe oryzae.Quantitative real time RT-PCR was used to investigate the expression profiles of various resistance-related genes in both the Pi36/AvPi36 incompatible interactions.Among 17 members of the OsMAPK family,OsMAPK2,OsMAPK4,OsMAPK8 and OsMAPK15 were differentially expressed,as were OsWRKY30,OsWRKY32,OsWRKY64 and OsWRKY67 among the 13 OsWRKY members studied.The induction of the expression of genes from both of these families suggests that both the JA and SA pathways are involved in Pi36-mediated defence.Other genes differentially expressed included the OsbZIP transcription factors,OsNIF1 and OsNIF2,and the NPR1 homologue OsNH2,all of which were reported in the rice blast pathosystem.The in-volvement of these genes illustrates the complexity of the downstream signalling pathways involved in the Pi36/AvrPi36 interaction.The resistance gene Pi36 confers a stable and high level of resistance against Chinese isolates of the rice blast pathogen Mag- naporthe oryzae. Quantitative real time RT-PCR was used to investigate the expression profiles of various resistance-related genes in both the Pi36/AvPi36 incompatible interactions. Among 17 members of the OsMAPK family, OsMAPK2, OsMAPK4, OsMAPK8 and OsMAPK15 were differentially expressed, as were OsWRKY30, OsWRKY32, OsWRKY64 and OsWRKY67 among the 13 OsWRKY members studied. The induction of the expression of genes from both of these families suggests that both the JA and SA pathways are involved in Pi36-mediated defence. Other genes differentially expressed included the OsbZIP transcription factors, OsNIF1 and OsN1F2, and the NPR1 homologue OsNH2, all of which were reported in the rice blast pathosystem. The in- volvement of these genes illustrates the complexity of the downstream signalling pathways involved in the Pi36/AvrPi36 interaction.

关 键 词：抗病相关基因 水稻稻瘟病 抗稻瘟病基因 基因差异表达 NPR1基因 PCR技术 抗性基因 稻瘟病菌 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]