The permeability effect of microcystin-RR on Escherichia coli and Bacillus subtilis  

作　　者：YANG CuiYun XIA ChuanHai ZHOU ShiWei LIU YongDing 

机构地区：[1]Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China [2]Shandong Key Laboratory for Eco-Environmental Science of Yellow River Delta, Binzhou 256600, China [3]Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

出　　处：《Chinese Science Bulletin》2010年第18期1894-1898,共5页

基　　金：supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (KZCX2-YW-Q07-04);the Foundation of Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology, SOA (200713);Open Research Fund Program of Shandong Key Laboratory for Eco-Environmental Science of Yellow River Delta, Binzhou China (2007KFJJ04)

摘　　要：Microcystins are a kind of cyclic hepatoxins produced by many species of cyanobacteria.Most previous work have been done on the toxic effects of microcystins on animals and plants.However,the reports about the effect of microcystins on microbial cells are very limited.In this work,the permeability of MC-RR on the cell outer membrane of Escherichia coli(E.coli) and Bacillus subtilis(B.subtilis) was discussed.The permeability effect of MC-RR on the cell outer membrane of E.coli and B.subtilis under different concentrations was demonstrated by a rapid and sustained reduction in the A675 values of lysozyme-treated cells.The decrease of the absorbance values showed a time-and dose-effect.The extravasations of protein and carbonhydrate increased with the increment of the treated-concentration of MC-RR.The results showed that MC-RR could increase the permeability of cell outer membranes of E.coli and B.subtilis.The synergistic effects of MC-RR and lysozyme on bacteria indicated that MC-RR might play an ecological role in bacteria in combination with other substances in some aquatic environments.Microcystins are a kind of cyclic hepatoxins produced by many species of cyanobacteria. Most previous work have been done on the toxic effects of microcystins on animals and plants. However, the reports about the effect of microcystins on microbial cells are very limited. In this work, the permeability of MC-RR on the cell outer membrane of Escherichia coli （E. coli） and Bacillus subtilis （B. subtilis） was discussed. The permeability effect of MC-RR on the cell outer membrane of E. coli and B. subtilis under different concentrations was demonstrated by a rapid and sustained reduction in the A675 values of lysozyme-treated cells. The decrease of the absorbance values showed a time- and dose-effect. The extravasations of protein and carbonhydrate increased with the increment of the treated-concentration of MC-RR. The results showed that MC-RR could increase the permeability of cell outer membranes of E. coli and B. subtilis. The synergistic effects of MC-RR and lysozyme on bacteria indicated that MC-RR might play an ecological role in bacteria in combination with other substances in some aquatic environments.

关 键 词：埃希氏大肠杆菌 枯草芽孢杆菌 微囊藻毒素 膜通透性 居民 微生物细胞 浓度增加 细胞通透性 

分 类 号：S917.1[农业科学—水产科学]