我国新分离盖塔病毒的部分基因组分子特征研究  

Partial genome molecular characteristics of Getah virus newly isolated in China

作　　者：陈维欣[1] 王环宇[2] 付士红[2] 李铭华[2] 刘桂芳[3] 姜红月[1] 王力华[2] 王海岩[3] 王志玉[1] 梁国栋[2] 

机构地区：[1]山东大学公共卫生学院病毒学研究室,济南250012 [2]中国疾病预防控制中心病毒病预防控制所病毒性脑炎室,北京100052 [3]山东省疾病预防控制中心

出　　处：《中华微生物学和免疫学杂志》2010年第5期399-404,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家科技部重大传染病专项（2003BA12A08-01）;艾滋病和病毒性肝炎等重大传染病防治重大专项项目（20087ZX10004-010）

摘　　要：目的 通过分子生物学方法研究我国山东省2008年分离的盖塔病毒（DY0824）基因组分子生物学特征.方法 应用逆转录聚合酶链反应（RT-PCR）扩增出结构区基因和3＇ UTR片段,连接到载体中进行序列测定,然后用Clustal X1.83和MegaAlign软件对测定的核苷酸和推测的氨基酸序列进行比较分析,用Mega4软件绘制系统发生树.结果 DY0824病毒株衣壳蛋白由804个核苷酸组成,编码268个氨基酸,与国内外其他分离株的核苷酸同源性为95.4%～99.9%,氨基酸同源性为97.4%～100%.E2蛋白全长1266个核苷酸,编码422个氨基酸,与其他株的核苷酸同源性为94.8%～99.5%,氨基酸同源性为97.6%～100%.该病毒3＇ UTR由401个核苷酸组成,存在3个重复序列.结论 山东省新分离盖塔病毒衣壳蛋白和E蛋白基国与该病毒原型分离株相比分别存在7个和10个氨基酸差异位点,病毒3＇UTR区域存在多个核苷酸差异位点.Objective To study the genome molecular characteristics of Getah virus（DY0824）which isolated in Shandong province,2008 by molecular biology methods.Methods Reverse transcriptasepolymerase chain reaction（RT-PCR）was used to amplify the structural gene and 3＇UTR fragments then the RT-PCR products were inserted into PGEM-T easy to be sequenced.Computer software was used to analyze the nucleotide and deduced amino acid sequence,and draw phylogenetic trees,including Clustal X1.83 and MegaAlign and Mega4.Results The capsid protein of DY0824 consists of 804 nucleotides,encoding 268 amino acids and the full-length of E2 protein is 1266 nucleotides,encoding 422 amino acids.The nucleotide homology of the capsid protein and the E2 protein with other strains were 95.4%-99.9%and 94.8%-99.5%,and the amino acid were 97.4%-100%and 97.6%-100%.The 3＇UTR of the virus include 401 nucleotides and there are three repeat sequence elements.Conclusion Compared with the prototype virus,the Getah virus isolated in Shandong province had 7 amino acid differences in capsid protein genes and 10 amino acid differences in E protein genes.The 3＇UTR region had multi-nucleotide changes.

关 键 词：盖塔病毒 衣壳蛋白基因 E2蛋白基因 3’非翻译区 核苷酸序列 

分 类 号：Q[生物学]