一起成人群体发热患者的病毒分离与鉴定  

Isolation and identification of the viral pathogen in an incident with crowd fever of adults

作　　者：胡燕[1] 白冰珂[1] 李伯安[1] 赵敏[1] 侯俊[1] 沈宏辉[1] 洪炜[1] 毛远丽[1] 貌盼勇[1] 

机构地区：[1]解放军第三零二医院传染病研究所,北京100039

出　　处：《中华微生物学和免疫学杂志》2010年第5期416-419,共4页Chinese Journal of Microbiology and Immunology

基　　金：军队“十一五”课题（06Q094）

摘　　要：目的从一起成人群体发热患者咽拭子标本中分离、鉴定病毒并研究其与疾病的关系。方法采集患者咽拭子标本，接种细胞分离病毒，以中和试验和分子生物学技术鉴定分离病毒；用分离的病毒制备抗原片，采用间接免疫荧光法检测患者血清中特异性IgM、IgG抗体。结果从10份发热患者咽拭子标本中分离到2株病毒，经核苷酸序列分析和中和试验证实为腺病毒11型；10例发热患者血清中，6例抗腺病毒11型特异性IgM阳性，3例特异性IgG阳性，1例特异性IgM、IgG均阴性。结论从患者咽拭子标本中分离到的腺病毒B组11型可能为引起此次群体发热的病原体。Objective To isolate and identify the causative agent in an incident with crowd fever of adults in Henan province. Methods The cells was inoculated by the throat swabs of the patients and followed by neutralization assay and other molecular methods. Then indirect immunofluorescence assay was performed to detect the specific IgM/IgG antibodies against virus in the serum of the patients. Results We have isolated 2 strain adenoviruses from 10 swab samples, which were both identified as Adll by sequence analysis and neutralization test. 6 of 10 samples were positive for IgM specific for adenovirus and 3 positive for IgG. The remaining sample was negative for both. Conclusion The causative agent in this incident with crowd fever of adults was Adl 1 in group B.

关 键 词：群体发热 病毒分离 鉴定 腺病毒 

分 类 号：R446[医药卫生—诊断学]