我国中部地区HIV感染者HLA Ⅰ类等位基因多态性与病毒载量的相关性研究  

Polymorphism of HLA class I alleles of HIV-infected individuals and its impact on HIV-1 viral load in central China

作　　者：贾明明[1] 洪坤学[1] 任国良[1] 郑扬[1] 郝晓培 刘真[1] 阮玉华[1] 赵全壁[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室,北京102206

出　　处：《中华微生物学和免疫学杂志》2010年第5期438-442,共5页Chinese Journal of Microbiology and Immunology

基　　金：＂十一五＂重大传染病防治研究项目（2008ZX10001-010-10）;科技部国际科技合作项目（2006DFA31510）

摘　　要：目的 分析我国中部地区既往采浆HIV感染者HLA Ⅰ类等位基因多态性及其与病毒载量的相关性.方法 应用PCR-SSP（序列特异性引物）技术对106例HIV感染者进行HLA Ⅰ类基因分型,分析HLA Ⅰ类等位基因多态性和单元型与血浆病毒载量的相关性.利用ELISPOT技术检测Gag蛋白特异性的CTL反应,并分析其与血浆病毒载量的相关性.结果 携带HLA Ⅰ类等位基因纯合子的感染者具有较高的病毒载量（P=0.0098）;HLA-A＊30、-B＊13、-Cw＊06、-Cw＊14等位基因及A＊30/B＊13/Cw＊06单元型与低病毒载量相关（P=0.0004,0.0103,0.0058,0.0371和0.0006）;携带HLA-A＊30/B＊13/Cw＊06单元型的感染者p2p7p1p6蛋白特异性的CTL反应与病毒载量负相关;携带HLA-Cw＊14的感染者p17蛋白特异性的CTL反应与病毒载量负相关.结论 我国中部地区既往采浆HIV感染人群HLA-A＊30、-B＊13、-Cw＊06、-Cw＊14等位基因及A＊30/B＊13/Cw＊06单元型携带者具有较低的病毒载量,其保护性与Gag蛋白特异性的CTL反应有关.Objective To explore the polymorphism of HLA class I alleles of HIV-infected former plasma donors and to investigate its impact on HIV-1 viral load in central China.Methods 106 subjects chronically infected with HIV-1 were recruited and HLA class I alleles were genotyped with PCR-SSP assay.HLA class I genotypes and haplotypes were determined and their association with plasma viral loads were analyzed.Gag-specific CTL responses were detected by an IFN-λ EUSPOT assay by using overlapping peptides,and their association with plasma viral loads were also analyzed.Results Subjects homozygous at HLA class I locus had higher plasma viral loads（P=0.0098）;HLA-A＊30,-B＊13,-Cw＊06,-Cw＊14 alleles and HLA-A＊30/B＊13/Cw＊06 haplotype were associated with lower plasma viral loads（P=0.0004,0.0103,0.0058,0.0371 and 0.0006）;an inverse correlation between p2p7p1p6-specific CTL responses and viral loads in subjects with HLA-A＊30/B＊13/Cw＊06 haplotype as well as an inverse correlation between p17-specific CTL responses and viral loads in subjects with HLA-Cw＊14 allele were observed.Conclusion HLA-A＊30,-B＊13,-Cw＊06,-Cw＊14 alleles and HLA-A＊30/B＊13/Cw＊06 haplotype were associated with lower plasma viral loads and Gag-specific CTL responses restricted by these HLA alleles may contribute to the protection.

关 键 词：人类免疫缺陷病毒 艾滋病 人类白细胞抗原 病毒载量 

分 类 号：R51[医药卫生—内科学]