大鼠内吗啡肽2和μ阿片受体参与内脏感觉和运动调控的形态学证据  

Morphological evidence for endomorphine 2 and μ opioid receptor involved in modulation of visceral sensation and movement in the rat

作　　者：李小莉[1,2] 牛乐[2] 吕伯昌[2] 张婷[2] 高晓群[1] 李云庆[2] 

机构地区：[1]郑州大学基础医学院人体解剖与组织胚胎学教研室,郑州450052 [2]第四军医大学人体解剖与组织胚胎学教研室暨梁辣琚脑研究中心,西安710032

出　　处：《神经解剖学杂志》2010年第3期233-238,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30771133,30971123)

摘　　要：目的:寻找内吗啡肽2(EM2)和μ阿片受体(MOR)在内脏感觉和运动调控过程中发挥作用的形态学证据。方法:(1)麦芽糖凝集素(WGA)示踪与免疫荧光组织化学染色技术相结合的多重标记方法;(2)用稀盐酸刺激胃,制作内脏痛动物模型;(3)免疫荧光组织化学多重染色方法。结果:(1)将WGA注入大鼠胃壁,在结状神经节(NG)内可见WGA标记神经元和WGA/EM2/MOR三标神经元;在孤束核(NTS)可见WGA跨节标记的纤维和终末以及WGA/EM2/MOR三标纤维和终末;在迷走神经运动背核(DMV)可见WGA逆标神经元和WGA/MOR双标神经元及其与EM2阳性纤维或终末形成的紧密接触。(2)将稀盐酸注入胃后,NG和NTS神经元内的FOS表达明显增多;在NG内可见EM2/FOS双标神经元;在NTS内可见EM2阳性纤维和终末与SP受体/FOS双标神经元形成紧密接触。结论:EM2和MOR可能影响内脏感觉和运动功能。Objective ： To look for morphological evidence for endomorphin 2 （EM2） and μ opioid receptor （MOR） exert modulatory effects on the visceral sensation and movement. Methods ： （ 1 ） Wheat-germ agglutinin （ WGA ） tracing combined with immunofluorescence histochemical staining; （2） Intragastric perfusion with low concentration of hydrochloric acid was used to make visceral pain model; （3） Immunofluorescence histochemical multi-staining. Results： （ 1 ） After injecting WGA into the stomach wall in the rat, WGA retrogradely labeled neurons and WGA/EM2/MOR triple-labeled neurons were observed in nodose ganglion （NG）. WGA transganglionically labeled fibers and terminals and WGA/EM2/ MOR triple-labeled fibers and terminals were observed in solitary tract nucleus （NTS）. WGA retrogradely labeled neu- rons and WGA/MOR double-labeled neurons and close contacts between WGA/MOR double-labeled neurons and EM2 immunopositive fibers and terminals were observed in dorsal motor nucleus of the vagus nerve （DMV）. （2） After intra- gastric perfusion with hydrochloric acid, FOS protein expression in neurons of the NG and NTS increased markedly ; EM2/ FOS double-labeled neurons were found in the NG; close contacts between substance P receptor （SPR）/FOS double labeled neurons and EM2 immunopositive fibers and terminals were observed in NTS. Conclusion： EM2 and MOR might be involved in modulation of the visceral sensation and movement.

关 键 词：内吗啡肽2 Μ阿片受体 内脏感觉 内脏运动 结状神经节 孤束核 迷走神经运动背核 大鼠 

分 类 号：R33[医药卫生—人体生理学]