内毒素血症大鼠脑底动脉内皮素-1能神经纤维表达与分布的变化  

The change of expression and distribution of endothelin-1 immunoreactive nerve fibers on cerebral arteries in endotoxemia rats

作　　者：万丽丹[1] 涂桂林[1] 林雪群[1] 

机构地区：[1]南昌大学医学院脑血管研究所解剖学教研室,南昌330006

出　　处：《神经解剖学杂志》2010年第3期289-293,共5页Chinese Journal of Neuroanatomy

基　　金：江西省自然科学基金项目(2009GZY0134)

摘　　要：目的:探讨内毒素血症大鼠脑底动脉内皮素-1(ET-1)能神经纤维诱发脑血管痉挛发生与发展的关系,为深入阐明内毒素血症诱发脑血管痉挛的发病机制提供形态学依据。方法:96只Wistar大鼠随机分为对照组、内毒素血症组(注射内毒素第3、6、12、24、48h),分别采用放射免疫法检测血浆内皮素-1水平的变化,采用免疫组织化学ABC法对大鼠脑底动脉内皮素-1能神经纤维进行观察。结果:内毒素血症后3、6、12h大鼠血浆内皮素-1水平较对照组明显升高(P<0.05),内毒素血症后24h和48h已趋于正常(P>0.05)。此外,对照组和内毒素组大鼠脑底动脉可见棕褐色,呈细线状分布的内皮素-1能免疫反应阳性纤维,其中内毒素血症后第6h和12h大鼠脑底动脉内皮素-1能神经纤维密度显著增高(P<0.05)。结论:内毒素血症大鼠血浆内皮素-1水平升高,脑底动脉内皮素-1能神经纤维密度增加,提示内皮素-1参与了内毒素血症诱发脑血管痉挛的病理生理过程,为脑血管痉挛的神经-体液调节机制提出了新思路,同时为临床预防和治疗脑血管痉挛提供了理论依据。Objective： To investigate the relationship between endotoxemia-induced cerebral vasospasm and endothelin- 1 （ ET-1 ） innervation on cerebral arteries, and to provide a morphological data for illuminating the pathogenesis of endo- toxemia-induced cerebral vasospasm in endotoxemia rats. Methods： 96 Wistar rats were randomly divided into control group and experimental group, the latter was then subdivided into 3, 6, 12, 24 and 48 h groups after the injection of lipopolysaccharide （LPS）. The change of plasma ET-1 was detected by radioimmunoassay technique, and ET-1 immunoreactive nerve fibers on cerebral arteries were examined with immunohistochemistry and measured with stereoscopy method. Results： LPS treatment caused a significant increase of plasma ET-1 at 3, 6 and 12 h after LPS injection when compared to the control group （ P 〈 0.05 ）. However in 24 h and 48 h LPS-treated groups, the plasma concentration of ET-1 decreased nearly to the normal level （ P 〉 0.05 ）. The ET-1 immunoreactive nerve fibers were observed in all groups, which were of brown color and thin. Moreover, at 6 h and 12 h after LPS treatment, the density of ET-1 immunoreaetive nerve fibers on cerebral arteries was much higher than that of control group （ P 〈 0.05 ）. Conclusion ： The endotoxemia induced the increase of ET-1 in blood plasma, and enhanced the density of ET-1 immunoreactive cerebral arterial nerve fibers. It indicated that ET-1 played an important role in the development of cerebral vasospasm induced by endotoxemia. The results provided a theoretical basis for the neurohumoral modulation mechanism of cerebral vasospasm.

关 键 词：内毒素血症 脑血管痉挛 脑底动脉 内皮素-1 大鼠 

分 类 号：R459.7[医药卫生—急诊医学]