盐胁迫对黄瓜根际土壤细菌群落结构和生长发育的影响  

Effects of Salt Stress on Rhizospheric Soil Bacterial Community Structure and Cucumber Yield

作　　者：吴凤芝[1] 包静[1] 刘淑芹[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030

出　　处：《园艺学报》2010年第5期741-748,共8页Acta Horticulturae Sinica

基　　金：国家‘973’项目(2009CB119004-05);国家自然科学基金项目(30771252);黑龙江省教育厅项目(1153018);黑龙江科技创新团队项目(2008)

摘　　要：以耐盐性不同的两个黄瓜品种'津春5号'(耐盐)和'津优1号'(盐敏感)为研究对象,设置4个NaCl浓度处理(0、100、200、300mmol·L-1),采用PCR-DGGE技术研究整个生长时期根际土壤细菌群落结构的变化。结果表明:盐胁迫能显著降低根际土壤细菌群落的丰富度(P<0.05),低盐处理下,盐敏感品种根际细菌的丰富度较高;高盐胁迫下,耐盐品种根际细菌群落的丰富度较高。高盐胁迫对盐敏感品种根际细菌群落结构组成影响较大。各时期DGGE图谱差异条带的序列片段经测序比对,推测为4大细菌类群:Firmicutes、Proteobacteria、Gemmatimonadetes和Bacteroidetes,且大多属于Proteobacteria(变形菌门)的α-变形菌纲和γ-变形菌纲。随着盐浓度的增加黄瓜的株高和单株产量依次降低。In order to study effects of salt stress on changes of soil microbial quantities and the diversity of soil microbial community by the technology of PCR-DGGE,two kinds of cucumbers with different saline tolerance were selected,Jinchun 5（salt-tolerant）and Jinyou 1（salt-sensitive）,and four concentrations of NaCl were designed（0,100,200 and 300 mmol · L^-1）.The results showed that salt stress could reduce the richness of soil bacterial community significantly（P0.05）.The richness of salt-sensitive cultivar was high under low salt treatment,while the richness of salt-tolerant cultivar was high under high salt treatment.High salt stress had more effects on the rhizosphere bacterial community structure of salt-tolerant cultivar.Sequences of different bands of four stages belonged to four bacterial groups,Firmicutes,Proteobacteria,Gemmatimonadetes and Bacteroidetes.Most of them belonged to Alphaproteobacteria and Gammaproteobacteria.With the increasing of salt concentration,the plant heights of cucumber and yield per plant reduced.

关 键 词：黄瓜 盐胁迫 根际土壤 细菌群落结构 多样性 

分 类 号：S642.2[农业科学—蔬菜学]